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主營產(chǎn)品: Flexcell細胞力學(xué)和regenhu細胞3D生物打印機銷售技術(shù)服務(wù): 美國Flexcell品牌FX-5000T細胞牽張應(yīng)力加載培養(yǎng)系統(tǒng),F(xiàn)X-5K細胞顯微牽張應(yīng)力加載培養(yǎng)系統(tǒng),Tissue Train三維細胞組織培養(yǎng)與測試系統(tǒng),F(xiàn)X-5000C三維細胞組織壓應(yīng)力加載培養(yǎng)系統(tǒng),STR-4000細胞流體剪切應(yīng)力加載培養(yǎng)系統(tǒng),德國cellastix品牌Optical Stretcher高通量單細胞牽引應(yīng)變與分析系統(tǒng) Regenhu品牌3D discovery細胞友好型3D生物打印機,piuma細胞納米壓痕測試分析、aresis多點力學(xué)測試光鑷,MagneTherm細胞腫瘤電磁熱療測試分析系統(tǒng)
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Nitric Oxide Measurement System ,一氧化氮測量系統(tǒng)

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  • 產(chǎn)品名稱:Nitric Oxide Measurement System ,一氧化氮測量系統(tǒng)
  • 產(chǎn)品型號:ArrowSTRAIGHT
  • 產(chǎn)品展商:Lazar世聯(lián)博研公司
  • 產(chǎn)品文檔:無相關(guān)文檔
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Nitric Oxide Measurement System ,一氧化氮測量系統(tǒng)Nitric Oxide Measurement System ,一氧化氮測量系統(tǒng)Measure NO in Your Smallest Samples Plasma Serum Urine Whole blood Saliva Tissue homogenates Cell Cultures

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Nitric Oxide Measurement System ,一氧化氮測量系統(tǒng)Nitric Oxide Measurement System ,一氧化氮測量系統(tǒng)Nitric oxide (NO) plays a major role in a variety of mammalian biological processes including blood pressure homeostasis, immune regulation, and nervous system signal transmission. NO is very unstable and has a physiological half life of only 1 to 40 seconds. Even though there are electrochemical (amperometric) methods for detecting NO, its rapid degradation to other nitrogen oxide compounds makes it difficult to determine quantitatively with a high degree of accuracy. The amperometric method for assay of NO is expensive, time consuming, and operator dependent. NO oxidizes into two stable end products, namely nitrite (NO2-) and nitrate (NO3-) ions. The concentration of these two end products can be used to quantify NO production without the measurement problems caused by the transient nature of NO.

There are various methods for quantitatively determining the concentration of both NO2- and NO3- ions. The most common methods involved the use of the Griess reagent which reacts with NO2- ion to produce a stable azo end product which is purple in color and can be quantified using colorimeteric or spectrophotometric analytical techniques. There are three basic problems with this colorimetric technique. First of all, the Griess reagent only reacts with the NO2- compound thereby requiring an additional difficult step to completely reduce NO3- ion to NO2-. Secondly, like with all colorimetric methods, the Griess technique can be affected by original sample color or turbidity. Thirdly, this method is very time consuming and tedious because it involved several intermediate chemical reactions.

Recently a potentially more accurate, less time consuming, and less tedious method has been developed for assaying both NO2- and NO3- which relies on the use of ion selective electrodes for both NO2- and NO3- ions. These electrodes have been miniaturized to be able to measure samples down to 50 to 100 microliters with detection limits down to the low microMolar range for both species. Since both NO2- and NO3- ions can be measured individually no reaction to reduce NO3- to NO2- is required. This micro electrode system comes complete with a sophisticated ion analyzer which attaches via RS232 or USB port to a PC or laptop to give accurate concentration measurements for both ions.Nitric Oxide Measurement System ,一氧化氮測量系統(tǒng)Nitric Oxide Measurement System ,一氧化氮測量系統(tǒng)Nitric Oxide Measurement System ,一氧化氮測量系統(tǒng)

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