biohelixH0300SbiohelixH0300SWhole Genome Amplification (WGA) techniques are a useful tool for cancer and genetic research, and typically used to amplify genomic DNA for archiving and down-stream analysis including genotyping, forensics, comparative genomic hybridization, and single cell analysis.
The Rapisome? pWGA kit non-specifically amplifies total DNA. Unlike other WGA products, the Rapisome? pWGA kit is based upon the in vitro reconstitution of naturally existing cellular DNA replication system, which performs fast isothermal DNA amplification without the need for thermocycling, prior heat-denaturation, or added primers. The pWGA technology uses a primase to synthesize primers on-site, generating multiple initiation sites for random, whole genome amplification. Therefore, there is no need to add random primers to the reaction.
The pWGA technology also utilizes a helicase to denature double-stranded template DNA, eliminating the initial heating step that is required by other WGA technologies. Thus, the Rapisome? pWGA reaction is truly isothermal.
pWGA is also faster than any other commercially available WGA techniques : the reaction time for pWGA is less than one hour. This makes Rapisome? pWGA attractive technology for use in diagnostic applications more so than other conventional methods.biohelixH0300S
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