Reaction condition:

1X Helicase Buffer M:
10 mM Dithiolthreitol
10 mM Magnesium Acetate
35 mM Tris-Acetate pH 7.5 @ 25°C

3 mM ATP.
Incubate at 37oC.

Notes:
E.coli UvrD helicase may require MutL and/or SSB for optimal performance in specific applications.

: MutL stimulates UvrD helicase activity more than tenfold by loading it onto the DNA substrate (Mechanic LE, Frankel BA, Matson SW (2000) J Biol Chem 275: 38337–38346).

: MutL and SSB are required in Helicase-Dependent Amplification using E. coli UvrD (Myriam Vincent, Yan Xu, and Huimin Kong (2004) EMBO reports Vol. 5, 795 - 800).

Storage Conditions:

Storage Temperature:
-20oC

Concentration:
0.5 mg/ml

Storage Buffer:
20 mM Tris-HCl pH8.3
200 mM NaCl
1.5 mM EDTA
25 mM ?-Mercaptoethanol
50% Glycerol

Quality Control:

Quality Assurance Statement:
E. coli UvrD Helicase is purified free of contaminating endonucleases and exonucleases. Each lot is tested for DNA unwinding activity.