Description:

Thermostable DNA Helicase is a DNA helicase that uses the energy generated by the hydrolysis of nucleoside triphosphates to break the hydrogen bonds linking the two strands together in duplex DNA. It is active in a temperature range of 45 to 65oC.

SUPPLIED WITH:
10X Annealing Buffer II
100 mM MgSO₄

Supplied by Users: ATP

Source:

Reaction condition:

1X Annealing Buffer II:
10 mM KCl
20 mM Tris-HCl, pH 8.8 @ 25°C

Supplement with 4 mM MgSO₄ and 3 mM ATP.
Incubate at 45 - 65oC.

Storage Conditions:

Quality Control:

Quality Assurance Statement:
Thermostable Helicase is purified free of contaminating endonucleases and exonucleases. Each lot is tested for DNA unwinding activity.