? 應(yīng)用于透性 (去膜) 心肌細(xì)胞的研究
? 測(cè)試kTR, 力-鈣離子濃度, 長(zhǎng)度-張力, 力-收縮速率和硬度等機(jī)械特性
? 備有8個(gè)溶液小槽的寛闊平臺(tái)以盛載不同濃度的鈣離子溶液
? 電動(dòng)化三維微定位裝置以準(zhǔn)確和快速地將樣本定位
? 可以配合標(biāo)準(zhǔn)顯微鏡或者倒置顯微鏡
? 可控溫度范圍: 0-40°C
1600A 是一個(gè)可靠和可重復(fù)性的實(shí)驗(yàn)測(cè)試系統(tǒng)，應(yīng)用于測(cè)試去膜心肌細(xì)胞的主動(dòng)和被動(dòng)性質(zhì)。透過(guò)使用其搖桿控制電動(dòng)化定位裝置可以輕易地令細(xì)胞的兩端分別連接于力傳感器和高速伺服電機(jī)，這大大提高了實(shí)驗(yàn)效能和簡(jiǎn)化了原本精細(xì)的樣本連接程序。
可控溫浴盆擁有8個(gè)溶液小槽令細(xì)胞可以輕易地在不同濃度的鈣離子溶液之間進(jìn)行轉(zhuǎn)換，該裝置會(huì)直接放置在倒置顯微鏡的置物平臺(tái)上進(jìn)行高清晰度的觀察和肌節(jié)間距的檢測(cè)?？蛇x擇附加一塊棱鏡去準(zhǔn)確地檢測(cè)出細(xì)胞于溶液槽的深度。
1400A測(cè)試系統(tǒng)可以控制和測(cè)試力度， 長(zhǎng)度和肌小節(jié)的間隔長(zhǎng)度(當(dāng)使用可附加的高速視頻肌節(jié)長(zhǎng)度系統(tǒng)) ，使其應(yīng)用范圍更廣泛， 從簡(jiǎn)單的力-鈣離子濃度曲線去到樣本完整的機(jī)械特性(例如kTR， 長(zhǎng)度-張力， 力-收縮速率和硬度) 都能夠被測(cè)試。附加的軟件程序(600A) 備有各種標(biāo)準(zhǔn)的實(shí)驗(yàn)方案去配合不同的實(shí)驗(yàn)需求。
完整系統(tǒng)包括:
●高速長(zhǎng)度操控儀
● 400A力傳感器
●電動(dòng)化三維微定位平臺(tái)
●帶有8個(gè)溶液小槽的可控溫平臺(tái)
●客制桌用電腦(附加螢?zāi)?: 配有控制和分析軟件系統(tǒng)
●溫度控制器和冷卻液循環(huán)器
●可附加肌小節(jié)間隔長(zhǎng)度檢測(cè)軟件(HVSL/ VSL)
應(yīng)用文獻(xiàn)：
一、心肌細(xì)胞和肌肉文獻(xiàn)
American Journal of …, 2005 - Am Physiological Soc
... Power output is linearly related to MyHC content in rat skinned myocytes and isolated working
hearts. ... were made using a capacitance-gauge transducer (model 403; sensitivity, 20 mV/mg plus
a x10 amplifier; resonant frequency, 600 Hz; Aurora Scientific; Aurora, Ontario ...
Cited by 45 - Related articles - Get at CISTI - All 5 versions
Altered single cell force-velocity and power properties in exercise-trained rat myocardium
[HTML] from physiology.org
GM Diffee… - Journal of Applied Physiology, 2003 - Am Physiological Soc
... The resulting skinned myocytes were resuspended in 8–10 ml of relaxing solution and kept on
ice throughout the days ... described previously (8). Skinned cardiac myocyte preparations were
attached between a capacitance-gauge transducer (model 403, Aurora Scientific) and a ...
Cited by 25 - Related articles - Get at CISTI - All 8 versions
Regional differences in effects of exercise training on contractile and biochemical properties of rat cardiac
myocytes
[HTML] from physiology.org
GM Diffee… - Journal of Applied Physiology, 2003 - Am Physiological Soc
... The resulting skinned myocytes were resuspended in 8–10 ml of relaxing solution and kept on
ice throughout the days ... described previously (9). Skinned cardiac myocyte preparations were
attached between a capacitance-gauge transducer (model 403, Aurora Scientific) and a ...
Cited by 24 - Related articles - Get at CISTI - All 5 versions
Force-length relations in isolated intact cardiomyocytes subjected to dynamic changes in mechanical load
[HTML] from physiology.org
G Iribe, M Helmes… - American Journal of Physiology- …, 2007 - Am Physiological Soc
... Gentaro Iribe, 1 Michiel Helmes, 1 ,2 and Peter Kohl 1. ... CCD, charge-coupled device. K was
measured directly, using a force transducer system (406A; Aurora Scientific, Aurora, ON, Canada)
factory calibrated for compression measurement, and mounted under a microscope. ...
Cited by 30 - Related articles - Get at CISTI - All 7 versions
Mouse intact cardiac myocyte mechanics: cross-bridge and titin-based stress in unactivated cells
[HTML] from rupress.org
…, N Harrell, CS Chung, M Helmes… - The Journal of …, 2011 - jgp.rupress.org
... Results were compared with those of previous studies on skinned myocytes (Helmes et al., 1996,
2003; Preetha et al., 2005) and ... fibers protruding from pipette tip), and their stiffness was determined
by cross-calibrating with a force transducer (model 406A; Aurora Scientific). ...
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Calcium sensitivity and the Frank-Starling mechanism of the heart are increased in titin N2B region-deficient mice
[HTML] from nih.gov
EJ Lee, J Peng, M Radke, M Gotthardt… - Journal of molecular and …, 2010 - Elsevier
... papillary muscles were dissected into small strips (cross-sectional area (CSA) 0.02 mm 2 ; length
1.2 mm) and small aluminum clips were glued to the ends of the muscle [33] in order to attach
the muscle at one end to a force transducer (model 406, Aurora Scientific) and at the ...
Cited by 5 - Related articles - All 6 versions
Analysing force–pCa curves
[HTML] from nih.gov
JS Walker, X Li… - Journal of muscle research and cell motility, 2010 - Springer
... Department of Medicine, University of Colorado Denver, Aurora, CO 80045, USA e-mail:
john.s.walker@ucdenver.edu ... contrast ability at magnifications up to 400 9 . Sarcomere length
was measured for each myocyte using a image based system (Aurora Scientific 901A HSVL ...
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Mouse intact cardiac myocyte mechanics: cross-bridge and titin-based stress in unactivated cells
[HTML] from rupress.org
NMP King, M Methawasin, J Nedrud… - The Journal of …, 2011 - jgp.rupress.org
... Next, 10 ml of myocyte stopping buffer 2 was added (perfusion buffer plus 0.05 ml BCS/ml buffer
and 12.5 μM CaCl 2 ... mm of carbon fibers protruding from pipette tip), and their stiffness was
determined by cross-calibrating with a force transducer (model 406A; Aurora Scientific). ...
Cited by 5 - Related articles - All 6 versions
Inorganic phosphate speeds loaded shortening in rat skinned cardiac myocytes
[HTML] from physiology.org
AC Hinken… - American Journal of Physiology- …, 2004 - Am Physiological Soc
... were introduced at one end of the preparation with a direct current (DC) torque motor (model
308; Aurora Scientific) driven by ... window In a new window. Table 2. Effects of P i addition on
mechanical properties of skinned cardiac myocyte preparations. ... 2. Araujo A and Walker JW. ...
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Protein Kinase A–Mediated Acceleration of the Stretch Activation Response in Murine Skinned Myocardium Is
Eliminated by Ablation of cMyBP-C
[HTML] from ahajournals.org
JE Stelzer, JR Patel… - Circulation research, 2006 - Am Heart Assoc
... The ends of the preparation were attached to the arms of a motor (model 312B, Aurora Scientific)
and force transducer (model 403, Aurora Scientific), as described earlier. ... Circ Res. 1998; 83:
471–480. Abstract/FREE Full Text. ? de Tombe PP, Stienen GJM. ...
Cited by 41 - Related articles - Get at CISTI - All 7 versions
Cycling cross-bridges increase myocardial stiffness at submaximal levels of Ca2+ activation
[HTML] from nih.gov
KS Campbell, JR Patel… - Biophysical journal, 2003 - Elsevier
... 913 ± 53 μm, width 130 ± 12 μm) were connected between a motor (Model 312B, time for
100-μm step 1 ms, Aurora Scientific, Ontario, Canada ... Many previous reports (Noble, 1977; Chiu
et al., 1982; de Tombe and ter Keurs, 1992; [Stuyvers et al., 1997] and [Stuyvers et al., 1998 ...
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Effect of stimulation rate, sarcomere length and Ca2+ on force generation by mouse cardiac muscle
[HTML] from physoc.org
BD Stuyvers, AD McCulloch, J Guo… - The Journal of …, 2002 - Physiological Soc
... The length of the muscle (ML) was set using a servo-controlled motor (model 318 B, Aurora
Scientific Inc., Aurora, ON, Canada) hooked to ... carbon fibre rod, constituted the force transducer
so that the resolution of the force measurement system was 0.5 μN(yùn) (see Stuyvers et al. ...
Cited by 45 - Related articles - Get at CISTI - All 10 versions
Effects of thin and thick filament proteins on calcium binding and exchange with cardiac troponin C
[HTML] from nih.gov
JP Davis, C Norman, T Kobayashi, RJ Solaro… - Biophysical journal, 2007 - Elsevier
... Aluminum T-clips were used to mount the trabecula between the arms of a high-speed
length controller (model 322C, Aurora Scientific, Ontario, Canada) and an isometric force
transducer (model 403A, Aurora Scientific) in relaxing solution. ...
Cited by 39 - Related articles - Get at CISTI - All 8 versions
Fabrication and Evaluation of Reconstructed Cardiac Tissue and Its Application to Bio-actuated Microdevices
H Horiguchi, K Imagawa, T Hoshino… - NanoBioscience, …, 2009 - ieeexplore.ieee.org
... D. Contractile Force Measurement of the Reconstructed Cardiac Tissue The contractile force of
the reconstructed cardiac tissue was measured using a force transducer. One end of the tissue
was fixed to a permeabilized fiber test apparatus (803B, Aurora Page 3. ...
Cited by 3 - Related articles - All 4 versions
The mechanoelectric feedback: a novel “calcium clamp” method, using tetanic contraction, for testing the role of
the intracellular free calcium
Y Yaniv, C Levy… - … of the New York Academy of …, 2006 - Wiley Online Library
... 308B, Aurora Scientific, Richmond Hill, Ontario, Canada) with a rise time of 250 μsec. ... from the
rat right ventricle (Spargue Dawley, 250–300 g). The dissection and the technique of mounting
the trabeculae in the experiment setup followed the procedures developed by ter Keurs. ...
Cited by 2 - Related articles - BL Direct - All 7 versions
Length dependence of force generation exhibit similarities between rat cardiac myocytes and skeletal muscle
fibres
[HTML] from physoc.org
LM Hanft… - The Journal of Physiology, 2010 - Wiley Online Library
... Length changes were introduced using a DC torque motor (model 308, Aurora Scientific, Inc.)
driven by voltage commands from a ... The kinetics of force redevelopment were obtained using
a procedure previously described for skinned cardiac myocyte preparations (Korte et al. ...
二、活體動(dòng)物肌肉機(jī)械特性測(cè)試文獻(xiàn)
Plasmid-based gene transfer in mouse skeletal muscle by electroporation
JD Schertzer, GS Lynch - METHODS IN MOLECULAR BIOLOGY- …, 2008 - Springer
... 1. The measurement of TA muscle function in situ requires a suitable platform (which is usually
Plexiglas) to secure the anesthetized mouse and for appropriate positioning of the servomotor. ...
Suitable systems are commer- cially available (Aurora Scientific, Aurora, ON, Canada). ...
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[HTML] < em> In Vivo</em> Canine Muscle Function Assay
[HTML] from jove.com
MK Childers, RW Grange… - Journal of Visualized …, 2011 - jove.com
... Stimulator, Grass, S88X, Stimulus isolation unit, Grass, SIU-C, Muscle lever system, Aurora
Scientific, 310C-LR, Footplate option. Signal interface, Aurora Scientific, Series 604A, Dynamic
Muscle Control & Analysis Software, Aurora Scientific, Aurora Scientific, ...
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Monitoring Murine Skeletal Muscle Function for Muscle Gene Therapy
[HTML] from nih.gov
CH Hakim, D Li, D Duan, CH Hakim, D Li… - Methods in molecular …, 2011 - Springer
... 1. Physiological saline (0.9% NaCl). 2. In situ muscle function assay system (Aurora
Scientific). The system consists of a 305C-LR dual mode transducer, a 701B stimulator,
a 604A signal interface, and an 809A footplate apparatus (Fig. 1c). ...
Androgen replacement therapy improves function in male rat muscles independently of hypertrophy and
activation of the Akt/mTOR pathway
C Hourde, C Jagerschmidt… - Acta …, 2009 - Wiley Online Library
... The right limbs were fixed with clamps (806A; Aurora Scientific, Aurora, Canada). The distal
tendons of the right soleus muscles were attached to a dual-mode lever arm system that measures
muscle isometric force (300C; Aurora Scientific) using a silk ligature. ...
Glutamine preserves skeletal muscle force during an inflammatory insult
BM Meador… - Muscle & nerve, 2009 - Wiley Online Library
... Force was measured using a dual-mode lever system, which controlled both length and
force/speed (305C-LR; Aurora Scientific). ... The ankle of the mouse was fixed in the servomotor's
footplate, with the talus (rotational center of the ankle joint) aligned with the servomotor axis. ...
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Sarcolipin overexpression in rat slow twitch muscle inhibits sarcoplasmic reticulum Ca2+ uptake and impairs
contractile function
[HTML] from jbc.org
AR Tupling, M Asahi… - Journal of Biological Chemistry, 2002 - ASBMB
... Three days after DNA injection, electrically evoked muscle force was assessed in situ from the
soleus muscle from each hindlimb across a range ... using a Grass S88 stimulator, and force data
were collected on-line using a 640A signal interface (Aurora Scientific Inc.) connected ...
Cited by 45 - Related articles - BL Direct - All 4 versions
Tibialis anterior muscles in mdx mice are highly susceptible to contraction-induced injury
[PDF] from 141.213.232.243
C Dellorusso, RW Crawford, JS Chamberlain… - Journal of muscle …, 2001 - Springer
... no. 85-23 (NIH), Revised 1985, Office of Science and Health reports, Bethesda, MD). In
situ preparation ... The tendon of the muscle was tied securely to the lever arm of a servomotor
(Model 305B-LR, Aurora Scientific, Richmond Hill, ON, Canada). ...