特點:
1.設計應用于肌纖維， 肌肉束，單條肌肉或細小的肌肉如trabeculae
2.適用于長度-張力， 力-收縮速率和硬度等的測量
?3.力度峰值達至100mN
4.可控溫度范圍: 0-40°C
5.400μL 或1900μL循環(huán)灌流浴， 包括兩條鉑電*用以提供刺激
6.可以配合標準顯微鏡或者倒置顯微鏡
7.可選擇單一等距實驗模式
概述：
1500A是一個一站式系統(tǒng)， 可以讓從事生理學研究方面 的科學家更簡單快捷地去測試細小組織的機械特性，例如*普遍的力度參量。
該系統(tǒng)的實驗裝置配合于絕大部份的倒置顯微鏡， 應用于成像觀察和螢光測量。實驗裝置擁有兩個不同大小的浴盤供以選擇去對應細小或是中等尺寸的樣本?？煽販卦∨鑲溆腥芤貉h(huán)灌流的裝置和兩條內置的鉑金電*以提供不同型態(tài)的電刺激。系統(tǒng)同時備有一個1L水庫為浴盆供應含氧媒體。
1500A 可以測試控制的力度和長度， 使其應用范圍更廣泛， 從簡單的張力測試去到樣本組織完整的機械特性(例如長度-張力，力-收縮速率和硬度)都能夠被測試。附加的軟件程序(600A) 備有各種標的實驗方案去配合不同的實驗需求。
力傳感器通過浴盆其中一端的小槽與樣本組織連接著， 該設計令浴盆的底部可以被有機玻璃密封， 從而非常適用于進行人工心肌組織或一些高氧耗量的研究。因為配置限制性很低， 1500A 是一個非常理想的測試系統(tǒng)去應用于細小肌肉組織方面的實驗。
完整的系統(tǒng)包括:
● 高速長度操控儀
● 400A力傳感器
● 高配置度的實驗浴盆
● 電場刺激器
●桌用電腦 (附加螢幕) ﹕ 配置控制和分析軟件系統(tǒng)
● 溫度控制器和冷卻液循環(huán)器
● 1升灌流水庫
● 可附加肌小節(jié)間隔長度檢測軟件(HVSL/ VSL)
六、1600A透性肌肉(去膜)細胞測試系統(tǒng)
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型號:1600A
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價格:請致電：010-67529703
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品牌:美國aurora
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1600A 透性肌肉細胞測試系統(tǒng)
特點:
? 應用于透性 (去膜) 心肌細胞的研究
? 測試kTR, 力-鈣離子濃度, 長度-張力, 力-收縮速率和硬度等機械特性
? 備有8個溶液小槽的寛闊平臺以盛載不同濃度的鈣離子溶液
? 電動化三維微定位裝置以準確和快速地將樣本定位
? 可以配合標準顯微鏡或者倒置顯微鏡
? 可控溫度范圍: 0-40°C
1600A 是一個可靠和可重復性的實驗測試系統(tǒng)，應用于測試去膜心肌細胞的主動和被動性質。透過使用其搖桿控制電動化定位裝置可以輕易地令細胞的兩端分別連接于力傳感器和高速伺服電機，這大大提高了實驗效能和簡化了原本精細的樣本連接程序。
可控溫浴盆擁有8個溶液小槽令細胞可以輕易地在不同濃度的鈣離子溶液之間進行轉換，該裝置會直接放置在倒置顯微鏡的置物平臺上進行高清晰度的觀察和肌節(jié)間距的檢測?？蛇x擇附加一塊棱鏡去準確地檢測出細胞于溶液槽的深度。
1400A測試系統(tǒng)可以控制和測試力度， 長度和肌小節(jié)的間隔長度(當使用可附加的高速視頻肌節(jié)長度系統(tǒng)) ，使其應用范圍更廣泛， 從簡單的力-鈣離子濃度曲線去到樣本完整的機械特性(例如kTR， 長度-張力， 力-收縮速率和硬度) 都能夠被測試。附加的軟件程序(600A) 備有各種標準的實驗方案去配合不同的實驗需求。
完整系統(tǒng)包括:
●高速長度操控儀
● 400A力傳感器
●電動化三維微定位平臺
●帶有8個溶液小槽的可控溫平臺
●客制桌用電腦(附加螢幕): 配有控制和分析軟件系統(tǒng)
●溫度控制器和冷卻液循環(huán)器
●可附加肌小節(jié)間隔長度檢測軟件(HVSL/ VSL)
應用文獻：
一、心肌細胞和肌肉文獻
American Journal of …, 2005 - Am Physiological Soc
... Power output is linearly related to MyHC content in rat skinned myocytes and isolated working
hearts. ... were made using a capacitance-gauge transducer (model 403; sensitivity, 20 mV/mg plus
a x10 amplifier; resonant frequency, 600 Hz; Aurora Scientific; Aurora, Ontario ...
Cited by 45 - Related articles - Get at CISTI - All 5 versions
Altered single cell force-velocity and power properties in exercise-trained rat myocardium
[HTML] from physiology.org
GM Diffee… - Journal of Applied Physiology, 2003 - Am Physiological Soc
... The resulting skinned myocytes were resuspended in 8–10 ml of relaxing solution and kept on
ice throughout the days ... described previously (8). Skinned cardiac myocyte preparations were
attached between a capacitance-gauge transducer (model 403, Aurora Scientific) and a ...
Cited by 25 - Related articles - Get at CISTI - All 8 versions
Regional differences in effects of exercise training on contractile and biochemical properties of rat cardiac
myocytes
[HTML] from physiology.org
GM Diffee… - Journal of Applied Physiology, 2003 - Am Physiological Soc
... The resulting skinned myocytes were resuspended in 8–10 ml of relaxing solution and kept on
ice throughout the days ... described previously (9). Skinned cardiac myocyte preparations were
attached between a capacitance-gauge transducer (model 403, Aurora Scientific) and a ...
Cited by 24 - Related articles - Get at CISTI - All 5 versions
Force-length relations in isolated intact cardiomyocytes subjected to dynamic changes in mechanical load
[HTML] from physiology.org
G Iribe, M Helmes… - American Journal of Physiology- …, 2007 - Am Physiological Soc
... Gentaro Iribe, 1 Michiel Helmes, 1 ,2 and Peter Kohl 1. ... CCD, charge-coupled device. K was
measured directly, using a force transducer system (406A; Aurora Scientific, Aurora, ON, Canada)
factory calibrated for compression measurement, and mounted under a microscope. ...
Cited by 30 - Related articles - Get at CISTI - All 7 versions
Mouse intact cardiac myocyte mechanics: cross-bridge and titin-based stress in unactivated cells
[HTML] from rupress.org
…, N Harrell, CS Chung, M Helmes… - The Journal of …, 2011 - jgp.rupress.org
... Results were compared with those of previous studies on skinned myocytes (Helmes et al., 1996,
2003; Preetha et al., 2005) and ... fibers protruding from pipette tip), and their stiffness was determined
by cross-calibrating with a force transducer (model 406A; Aurora Scientific). ...
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Calcium sensitivity and the Frank-Starling mechanism of the heart are increased in titin N2B region-deficient mice
[HTML] from nih.gov
EJ Lee, J Peng, M Radke, M Gotthardt… - Journal of molecular and …, 2010 - Elsevier
... papillary muscles were dissected into small strips (cross-sectional area (CSA) 0.02 mm 2 ; length
1.2 mm) and small aluminum clips were glued to the ends of the muscle [33] in order to attach
the muscle at one end to a force transducer (model 406, Aurora Scientific) and at the ...
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Analysing force–pCa curves
[HTML] from nih.gov
JS Walker, X Li… - Journal of muscle research and cell motility, 2010 - Springer
... Department of Medicine, University of Colorado Denver, Aurora, CO 80045, USA e-mail:
john.s.walker@ucdenver.edu ... contrast ability at magnifications up to 400 9 . Sarcomere length
was measured for each myocyte using a image based system (Aurora Scientific 901A HSVL ...
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Mouse intact cardiac myocyte mechanics: cross-bridge and titin-based stress in unactivated cells
[HTML] from rupress.org
NMP King, M Methawasin, J Nedrud… - The Journal of …, 2011 - jgp.rupress.org
... Next, 10 ml of myocyte stopping buffer 2 was added (perfusion buffer plus 0.05 ml BCS/ml buffer
and 12.5 μM CaCl 2 ... mm of carbon fibers protruding from pipette tip), and their stiffness was
determined by cross-calibrating with a force transducer (model 406A; Aurora Scientific). ...
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Inorganic phosphate speeds loaded shortening in rat skinned cardiac myocytes
[HTML] from physiology.org
AC Hinken… - American Journal of Physiology- …, 2004 - Am Physiological Soc
... were introduced at one end of the preparation with a direct current (DC) torque motor (model
308; Aurora Scientific) driven by ... window In a new window. Table 2. Effects of P i addition on
mechanical properties of skinned cardiac myocyte preparations. ... 2. Araujo A and Walker JW. ...
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Protein Kinase A–Mediated Acceleration of the Stretch Activation Response in Murine Skinned Myocardium Is
Eliminated by Ablation of cMyBP-C
[HTML] from ahajournals.org
JE Stelzer, JR Patel… - Circulation research, 2006 - Am Heart Assoc
... The ends of the preparation were attached to the arms of a motor (model 312B, Aurora Scientific)
and force transducer (model 403, Aurora Scientific), as described earlier. ... Circ Res. 1998; 83:
471–480. Abstract/FREE Full Text. ? de Tombe PP, Stienen GJM. ...
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Cycling cross-bridges increase myocardial stiffness at submaximal levels of Ca2+ activation
[HTML] from nih.gov
KS Campbell, JR Patel… - Biophysical journal, 2003 - Elsevier
... 913 ± 53 μm, width 130 ± 12 μm) were connected between a motor (Model 312B, time for
100-μm step 1 ms, Aurora Scientific, Ontario, Canada ... Many previous reports (Noble, 1977; Chiu
et al., 1982; de Tombe and ter Keurs, 1992; [Stuyvers et al., 1997] and [Stuyvers et al., 1998 ...
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Effect of stimulation rate, sarcomere length and Ca2+ on force generation by mouse cardiac muscle
[HTML] from physoc.org
BD Stuyvers, AD McCulloch, J Guo… - The Journal of …, 2002 - Physiological Soc
... The length of the muscle (ML) was set using a servo-controlled motor (model 318 B, Aurora
Scientific Inc., Aurora, ON, Canada) hooked to ... carbon fibre rod, constituted the force transducer
so that the resolution of the force measurement system was 0.5 μN (see Stuyvers et al. ...
Cited by 45 - Related articles - Get at CISTI - All 10 versions
Effects of thin and thick filament proteins on calcium binding and exchange with cardiac troponin C
[HTML] from nih.gov
JP Davis, C Norman, T Kobayashi, RJ Solaro… - Biophysical journal, 2007 - Elsevier
... Aluminum T-clips were used to mount the trabecula between the arms of a high-speed
length controller (model 322C, Aurora Scientific, Ontario, Canada) and an isometric force
transducer (model 403A, Aurora Scientific) in relaxing solution. ...
Cited by 39 - Related articles - Get at CISTI - All 8 versions
Fabrication and Evaluation of Reconstructed Cardiac Tissue and Its Application to Bio-actuated Microdevices
H Horiguchi, K Imagawa, T Hoshino… - NanoBioscience, …, 2009 - ieeexplore.ieee.org
... D. Contractile Force Measurement of the Reconstructed Cardiac Tissue The contractile force of
the reconstructed cardiac tissue was measured using a force transducer. One end of the tissue
was fixed to a permeabilized fiber test apparatus (803B, Aurora Page 3. ...
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The mechanoelectric feedback: a novel “calcium clamp” method, using tetanic contraction, for testing the role of
the intracellular free calcium
Y Yaniv, C Levy… - … of the New York Academy of …, 2006 - Wiley Online Library
... 308B, Aurora Scientific, Richmond Hill, Ontario, Canada) with a rise time of 250 μsec. ... from the
rat right ventricle (Spargue Dawley, 250–300 g). The dissection and the technique of mounting
the trabeculae in the experiment setup followed the procedures developed by ter Keurs. ...
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Length dependence of force generation exhibit similarities between rat cardiac myocytes and skeletal muscle
fibres
[HTML] from physoc.org
LM Hanft… - The Journal of Physiology, 2010 - Wiley Online Library
... Length changes were introduced using a DC torque motor (model 308, Aurora Scientific, Inc.)
driven by voltage commands from a ... The kinetics of force redevelopment were obtained using
a procedure previously described for skinned cardiac myocyte preparations (Korte et al. ...
二、活體動物肌肉機械特性測試文獻
Plasmid-based gene transfer in mouse skeletal muscle by electroporation
JD Schertzer, GS Lynch - METHODS IN MOLECULAR BIOLOGY- …, 2008 - Springer
... 1. The measurement of TA muscle function in situ requires a suitable platform (which is usually
Plexiglas) to secure the anesthetized mouse and for appropriate positioning of the servomotor. ...
Suitable systems are commer- cially available (Aurora Scientific, Aurora, ON, Canada). ...
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[HTML] < em> In Vivo</em> Canine Muscle Function Assay
[HTML] from jove.com
MK Childers, RW Grange… - Journal of Visualized …, 2011 - jove.com
... Stimulator, Grass, S88X, Stimulus isolation unit, Grass, SIU-C, Muscle lever system, Aurora
Scientific, 310C-LR, Footplate option. Signal interface, Aurora Scientific, Series 604A, Dynamic
Muscle Control & Analysis Software, Aurora Scientific, Aurora Scientific, ...
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Monitoring Murine Skeletal Muscle Function for Muscle Gene Therapy
[HTML] from nih.gov
CH Hakim, D Li, D Duan, CH Hakim, D Li… - Methods in molecular …, 2011 - Springer
... 1. Physiological saline (0.9% NaCl). 2. In situ muscle function assay system (Aurora
Scientific). The system consists of a 305C-LR dual mode transducer, a 701B stimulator,
a 604A signal interface, and an 809A footplate apparatus (Fig. 1c). ...
Androgen replacement therapy improves function in male rat muscles independently of hypertrophy and
activation of the Akt/mTOR pathway
C Hourde, C Jagerschmidt… - Acta …, 2009 - Wiley Online Library
... The right limbs were fixed with clamps (806A; Aurora Scientific, Aurora, Canada). The distal
tendons of the right soleus muscles were attached to a dual-mode lever arm system that measures
muscle isometric force (300C; Aurora Scientific) using a silk ligature. ...
Glutamine preserves skeletal muscle force during an inflammatory insult
BM Meador… - Muscle & nerve, 2009 - Wiley Online Library
... Force was measured using a dual-mode lever system, which controlled both length and
force/speed (305C-LR; Aurora Scientific). ... The ankle of the mouse was fixed in the servomotor's
footplate, with the talus (rotational center of the ankle joint) aligned with the servomotor axis. ...
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Sarcolipin overexpression in rat slow twitch muscle inhibits sarcoplasmic reticulum Ca2+ uptake and impairs
contractile function
[HTML] from jbc.org
AR Tupling, M Asahi… - Journal of Biological Chemistry, 2002 - ASBMB
... Three days after DNA injection, electrically evoked muscle force was assessed in situ from the
soleus muscle from each hindlimb across a range ... using a Grass S88 stimulator, and force data
were collected on-line using a 640A signal interface (Aurora Scientific Inc.) connected ...
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Tibialis anterior muscles in mdx mice are highly susceptible to contraction-induced injury
[PDF] from 141.213.232.243
C Dellorusso, RW Crawford, JS Chamberlain… - Journal of muscle …, 2001 - Springer
... no. 85-23 (NIH), Revised 1985, Office of Science and Health reports, Bethesda, MD). In
situ preparation ... The tendon of the muscle was tied securely to the lever arm of a servomotor
(Model 305B-LR, Aurora Scientific, Richmond Hill, ON, Canada). ...
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