• Easy to Use, Very Cost Effective 
• Low Noise, High Speed 
• Can do Patch or Whole Cell with optional Dual Range PC-ONE-60 Remote Switchable Headstage 
• Built in Leakage Subtraction 
• Offsets up to +/-300mV 
• 3-1/2 Digit LCD Panel Meter 
• Telegraphs for GAIN and FILTER 
• Eight Output Gains 

• Auto Zero with SEARCH and HOLD capability 
• Two Value Test Generator forpipette and seal resistance 
• Unfiltered and Filtered Im Outputs 
• Five position 3-pole Low Pass Bessel Filter 
• 10 Megohm Headstage for Large Whole Cells 
• Popular 50:1 and 10:1 Command Inputs 
• Voltage/Current or Zero Current Mode from External Control 

• Large Whole Cell, 10 Megohm , (to 1000nA) (Model # PC-ONE-10) 
• Patch, 10 Gigohm, (to 1nA) (Model #PC-ONE-40)  
• Whole Cell, 100 Megohm, (to 100nA) (Model #PC-ONE-20) 
• Bilayer, 10 Gigohm, (Model #PC-ONE-50) 
• Small Whole Cell, 1 Gigohm, (to 10nA) (Model #PC-ONE-30) 
• Whole Cell/Patch, 100Megohm/10Gigohm Dual Range, (Model #PC-ONE-60) 
• Voltammetry, 2 volt Command for (10Megohm through 10 Gigohm)(Model #PC-ONE-70) 

Planar Lipid Bilayer Recording A special Bilayer headstage (10Gigohm feedback resistor) not only allows applications to 500pF Bilayers, but it also allows you to "short" the Bilayer during the onset of a step command to "quick charge" the Bilayer capacitance. A unique 10Gigohm (Bilayer) position on the Headstage Selector switch modifies the Capacitance Compensation from the normal 10pF Patch application to the 500pF Bilayer application.

Voltammetry (Electrochemical) Applications A special headstage with a +/-2 volt command range allows the PC-ONE to "voltage clamp"a carbon-fiber electrode so that extracellular concentrations of various neurotransmitters can be measured at any specified oxidation/reduction potential. Headstages can be specified with 10 Megohm, 100 Megohm, 1 Gigohm or 10 Gigohm feedback sensing resistors (i.e., 1000nA down to 1nA full scale sensitivities).

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Whole Cell Voltage and Current Clamping The PC-ONE handles three ranges of whole cell headstages for currents up to 1000nA (10 Megohm feedback). Standard LEAKAGE subtraction/compensation allows you to quickly adjust for leak errors on-line during an experiment.

• Noise: (PC-ONE-40 10Gigohm headstage)1.0kHz Bandwidth, 0.06pArms; 3.0kHz Bandwidth, 0.10pArms;5.0kHz Bandwidth, 0.16pArms; 10kHz Bandwidth, 0.30pArms 
• Capacity Compensation: Fast: 0 to 1µs, Slow 0 to 1ms (Patch) 
• Cap Comp Range:10pF Patch; 100pF Whole Cell; 500pF Bilayer 
• Series Resistance Compensation: 0 to 100Mohm, ten turn dial (all whole cell ranges) 
• Filter:100, 300, 1K, 3K, 10kHz Low Pass Bessel Filter (3 pole) 
• Gain: .1, .2, .5, 1, 2, 5, 10, 20 amplification (b)of Im signal with headstage scale factor (a) of 0.1, 1, 10, 100 for sensitivities up to 2,000mV/pA (Patch) (Scale factor (a) is shown on convenient LED's next to the GAIN switch) 
• Junction Potential Correction: (3 types, all +300mV) Manual-ten turn control; Search- continuously corrects potential with a slow "attack" time; Hold- correction potential only when UPDATE switch is pushed 
• TEST Commands:TESTswitch with 0.2mV and 20mVpositions for pipette and seal resistance forming seal (2pA and 200pA Current Clamp) 
• Holding Potential Command:+200mV ten turn control with ON/OFFswitch (+2nA Current Clamp) 
• Headstage Dimensions: 7.7cm (2.2") x 3.5cm (1.3") x 2.8cm (1.1"); detachable mounting rod is 0.62cm (1/4") diameter by 7.6cm (3")long. Cable length is 1.8m (6') 
• Mainframe Dimensions: 9cm (3-1/2") high, 48cm (19") wide, 25cm (10")deep. Supplied with rack mounting hardware 
• Step Command Input: 50:1 (i.e., 10 volts gives 200mV) industry standard, and 10:1 with center OFF (10Kohm input, 10 volts max) (5V/nA or 1V/nA Current Clamp) 
• High Frequency Boost: Automatic with no need for confusing ON/OFFswitch 
• Panel Meter: 3-1/2 digit LCD with 5 functions and automatic decimal point 

• COMD IN - the command at the command input (1999mV) 
• Vhold - the holding potential (1999mV) 
• Vtot comd - the total of all commands presented to the pipette (1999mV) 
• Vm - the membrane voltage (1999mV) 
• Im - the membrane current before processing: 199.9pA 10Gigohm headstage; 19.99pA100Megohm headstage 

• Computer Telegraph BNCOutputs: GAINand FILTER, analog 0.4 volts per step 
• External Mode Select: From Izero Mode, 5 volt CMOS input allows either Vclamp or Iclamp selection via I/O15-pin connector 
• Membrane Current (Im) Output: 

• Im: Unfiltered, Im signal. Sensitivity=(a)(b)mV/pA (used to record data unfiltered) 
• FILTERED Im: Filtered Im signal. Sensitivity (a)(b)mV/pA (used to view data) 

• Sample & Hold Option: TTL activated Sample & Hold on Im path via back panel BNC 
• Electrode Holder: (order separately) Low noise polycarbonate with 1mm pressure port and BNC sized (ie., industry standard) end.  HB180- accepts glass 1.0 to 1.8mm OD (silver wire internal) 
• Power: 100/115 or 230 VAC 50/60 Hz (15 watts) (specify voltage) 

Other Useful Accessories: I/OCable; Prism Pipette Glass; FL-4 Filter; Zeitz Pipette Puller;

Headstage Probes

Accessories