微滴單細(xì)胞包裝系統(tǒng)

簡(jiǎn)介：

我們的半自動(dòng)系統(tǒng)將單細(xì)胞或生物分子包裝進(jìn)微滴中，為后續(xù)的篩選和分析做準(zhǔn)備。它的包裝效率可以達(dá)到每秒70000個(gè)微滴，且流速可以嚴(yán)格控制。這臺(tái)設(shè)備使得快速且敏感地檢測(cè)微滴中單個(gè)細(xì)胞分泌的或者細(xì)胞相關(guān)的蛋白成為可能。

這一包裝系統(tǒng)不影響細(xì)胞活性，由于微滴的大小和體積有很大范圍的可變性，因而能夠用于不同大小的細(xì)胞類(lèi)型。這些微滴被新式的表面活性劑穩(wěn)定，細(xì)胞能夠在其中生長(zhǎng)，甚至能培養(yǎng)或保存許多天。

主要特點(diǎn)：

·半自動(dòng)微滴生成器

·在微滴中包裝單個(gè)細(xì)胞或生物分子

·高速生成微滴（高達(dá)70000/秒）

·可同時(shí)在微滴中摻入探針和細(xì)胞，使得能夠敏感地檢測(cè)到分泌蛋白（例如抗體，生長(zhǎng)因子，細(xì)胞因子，酶等）

·用戶(hù)決定微流體流動(dòng)速率

·微滴生成的光學(xué)成像經(jīng)過(guò)嚴(yán)格的質(zhì)量管理/測(cè)試（QA/QC）

·大范圍的微滴大小和體積

應(yīng)用舉例：

·生物**的發(fā)現(xiàn)：從初始漿細(xì)胞（B細(xì)胞或雜交瘤細(xì)胞）中發(fā)現(xiàn)抗體或轉(zhuǎn)錄產(chǎn)物；

·生物加工：快速鑒定和分離高表達(dá)克?。?/span>

·診斷：探測(cè)并測(cè)試循環(huán)腫瘤和其他**相關(guān)細(xì)胞；

·抗藥性研究：從大量的微生物或腫瘤細(xì)胞集群中鑒定和分離稀有的耐藥細(xì)胞；

·酶的進(jìn)化：篩選數(shù)百萬(wàn)酶結(jié)構(gòu)以選擇*高效的突變體；

·合成生物學(xué)：研究工程微生物庫(kù)中產(chǎn)生的大量有價(jià)值的分子。

技術(shù)參數(shù)：

1.       Abalde-Cela et al. 2015. High-throughput detection of ethanol-producing cyanobacteria in a microdroplet platform. J. R. Soc. Interface 12: 2015.0216

2.       Bakewell et al. 2015. Information processing tools for extracting the electrical properties of nanoparticles. AIP Conf. Proc. 1646, 17-24

3.       Bakewell et al. Exploring and Evaluating Micro-environment and Nanoparticle Dielectrophoretic-induced Interactions with Image Analysis Methods. Materials Today: Proceedings, 867-874, 3(3) 2016.

4.       Chokkalingam et al. 2013. Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics. Lab Chip 13: 4740-4744

5.       Holmes et al. 2014. Separation of blood cells with differing deformability using deterministic lateral displacement. Interface Focus 4. 20140011

6.       Kruger et al. 2014. Deformability-based red blood cell separation in deterministic lateral displacement devices—A simulation study. Biomicrofluidics 8; 054114

7.       Ma et al. 2012. Fabrication of Microgel Particles with Complex Shape via Selective Polymerization of Aqueous Two-Phase Systems. Small. 8(15): 2356-2360

8.  Ma et al. 2013. Monodisperse collagen–gelatin beads as potential platforms for 3D cell culturing. J. Mater. Chem. B, 1; 5128-5136

9.Salmon et al. 2016. Monitoring early-stage nanoparticle assembly in microdroplets by optical spectroscopy and SERS. Small. Doi:10.1002/smll.201503513

10. Sherwood et al. 2014. Spatial Distributions of Red Blood Cells Significantly Alter Local Haemodynamics. PLOS One 9(6): :e100473

11. Shim et al. 2013. Ultrarapid Generation of Femtoliter Microfluidic Droplets for Single-Molecule-Counting Immunoassays. ACS Nano 7(7): 5955-5964

12. Smith et al. 2013. Sensitive, High Throughput Detection of Proteins in Individual, Surfactant-Stabilized Picoliter Droplets Using Nanoelectrospray Ionization Mass Spectrometry. Analytical Chemistry. 85(8): 3812-3816

13. Parker, R. M. et al. 2015. Electrostatically directed self-assembly of ultrathin supramolecular polymer microcapsules. Advanced Functional Materials. 25(26): 4091-4100.

14. Use of standards for digital biological information in the design, construction and description of a synthetic biological system – Guide. 2015. PAS 246:2015

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