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細(xì)胞毒性檢測系統(tǒng)

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  • 產(chǎn)品名稱:細(xì)胞毒性檢測系統(tǒng)
  • 產(chǎn)品型號:Dimscan
  • 產(chǎn)品展商:Dimscan,bioimagingsolutions
  • 產(chǎn)品文檔:無相關(guān)文檔
簡單介紹

DIMSCAN是用于在組織培養(yǎng)板中定量的相對細(xì)胞數(shù)半自動數(shù)字圖像顯微術(shù)系統(tǒng)。使用熒光素二乙酸酯(FDA)通過DIMSCAN測定細(xì)胞毒性測定法中,染料在活細(xì)胞選擇性地累積,利用集落形成測定法,可以實(shí)現(xiàn)動態(tài)范圍在4至7天內(nèi)的生長曲線。 (1)活細(xì)胞的相對數(shù)目是通過測算熒光素二乙酸酯(FDA)的熒光強(qiáng)度,削減由背景熒光的閾值和由曙紅所終止的不可存活的細(xì)胞的熒光值進(jìn)行測量。 (2)可以使用含6至38

產(chǎn)品描述

A New Generation of Fluorescence Digital Image Microscopy System for Measuring Cytotoxicity in Microplate

DIMSCAN自動熒光數(shù)字圖像顯微處理系統(tǒng),DIMSCAN細(xì)胞毒性測定系統(tǒng),DIMSCAN活細(xì)胞的相對數(shù)測定系統(tǒng),DIMSCAN**測定(**、病毒、**、病原體)系統(tǒng),DIMSCAN蛋白質(zhì)組檢測系統(tǒng),DIMSCAN核酸測定系統(tǒng),DNA和基因組檢測;快速即時(shí)的臨床診斷;食品和環(huán)境檢測;**檢測



圖1 DIMSCAN觀測系統(tǒng)外觀

2、系統(tǒng)亮點(diǎn)概述 
DIMSCAN是用于在組織培養(yǎng)板中定量的相對細(xì)胞數(shù)半自動數(shù)字圖像顯微術(shù)系統(tǒng)。使用熒光素二乙酸酯(FDA)通過DIMSCAN測定細(xì)胞毒性測定法中,染料在活細(xì)胞選擇性地累積,利用集落形成測定法,可以實(shí)現(xiàn)動態(tài)范圍在4至7天內(nèi)的生長曲線。

(1)活細(xì)胞的相對數(shù)目是通過測算熒光素二乙酸酯(FDA)的熒光強(qiáng)度,削減由背景熒光的閾值和由曙紅所終止的不可存活的細(xì)胞的熒光值進(jìn)行測量。 
(2)可以使用含6至384個(gè)孔的矩形培養(yǎng)板,對于96孔板平均掃描時(shí)間為6分鐘。 
(3)系統(tǒng)采用了基于自相關(guān)函數(shù)的自動聚焦功能以*大限度地提高了系統(tǒng)的自動化的程度。 
(4)相關(guān)程序存儲重新掃描過程中的圖像,并在用戶掃描結(jié)束時(shí)顯示出來,至今進(jìn)行新的掃描過程。 
(5)具有操作簡便、準(zhǔn)確、靈敏、重復(fù)性好等優(yōu)點(diǎn),細(xì)胞數(shù)量在1, 10, 20, 50, 100, 200, 500, 1000, 2000, 5000和10000 每個(gè)培養(yǎng)孔,測量8個(gè)重復(fù),細(xì)胞數(shù)量與熒光強(qiáng)度呈良好的線性關(guān)系,線性相關(guān)系數(shù)為0.9996746375
DIMSCAN自動熒光數(shù)字圖像顯微處理系統(tǒng),DIMSCAN細(xì)胞毒性測定系統(tǒng),DIMSCAN活細(xì)胞的相對數(shù)測定系統(tǒng),DIMSCAN**測定(**、病毒、**、病原體)系統(tǒng),DIMSCAN蛋白質(zhì)組檢測系統(tǒng),DIMSCAN核酸測定系統(tǒng),DNA和基因組檢測;快速即時(shí)的臨床診斷;食品和環(huán)境檢測;**檢測
圖2細(xì)胞TC-71(尤因氏肉瘤)3天,20000細(xì)胞

DIMSCAN自動熒光數(shù)字圖像顯微處理系統(tǒng),DIMSCAN細(xì)胞毒性測定系統(tǒng),DIMSCAN活細(xì)胞的相對數(shù)測定系統(tǒng),DIMSCAN**測定(**、病毒、**、病原體)系統(tǒng),DIMSCAN蛋白質(zhì)組檢測系統(tǒng),DIMSCAN核酸測定系統(tǒng),DNA和基因組檢測;快速即時(shí)的臨床診斷;食品和環(huán)境檢測;**檢測
圖3 96孔板測試系統(tǒng)熒光測量線性(r2 = 0.9996746375) 
3、適用范圍 
(1)細(xì)胞毒性試驗(yàn),通過DIMSCAN測量集落形成可以實(shí)現(xiàn)動態(tài)范圍在4至7天內(nèi)的生長曲線。

DIMSCAN自動熒光數(shù)字圖像顯微處理系統(tǒng),DIMSCAN細(xì)胞毒性測定系統(tǒng),DIMSCAN活細(xì)胞的相對數(shù)測定系統(tǒng),DIMSCAN**測定(**、病毒、**、病原體)系統(tǒng),DIMSCAN蛋白質(zhì)組檢測系統(tǒng),DIMSCAN核酸測定系統(tǒng),DNA和基因組檢測;快速即時(shí)的臨床診斷;食品和環(huán)境檢測;**檢測

DIMSCAN自動熒光數(shù)字圖像顯微處理系統(tǒng),DIMSCAN細(xì)胞毒性測定系統(tǒng),DIMSCAN活細(xì)胞的相對數(shù)測定系統(tǒng),DIMSCAN**測定(**、病毒、**、病原體)系統(tǒng),DIMSCAN蛋白質(zhì)組檢測系統(tǒng),DIMSCAN核酸測定系統(tǒng),DNA和基因組檢測;快速即時(shí)的臨床診斷;食品和環(huán)境檢測;**檢測
圖4細(xì)胞毒性試驗(yàn)
Cell line TC-71 (Ewing's sarcoma), day 3, 20000 cells
Treated with 4-HPR + n-oleylethanolamine (nOE), 1:1 (0-12 um both)

(2)熒光掃描圖象處理 
DIMSCAN自動熒光數(shù)字圖像顯微處理系統(tǒng),DIMSCAN細(xì)胞毒性測定系統(tǒng),DIMSCAN活細(xì)胞的相對數(shù)測定系統(tǒng),DIMSCAN**測定(**、病毒、**、病原體)系統(tǒng),DIMSCAN蛋白質(zhì)組檢測系統(tǒng),DIMSCAN核酸測定系統(tǒng),DNA和基因組檢測;快速即時(shí)的臨床診斷;食品和環(huán)境檢測;**檢測
圖5 圖象閾值處理(左邊:原始圖像;右邊:處理后的二值圖像)

3)建立細(xì)胞熒光預(yù)覽縮略圖 
DIMSCAN自動熒光數(shù)字圖像顯微處理系統(tǒng),DIMSCAN細(xì)胞毒性測定系統(tǒng),DIMSCAN活細(xì)胞的相對數(shù)測定系統(tǒng),DIMSCAN**測定(**、病毒、**、病原體)系統(tǒng),DIMSCAN蛋白質(zhì)組檢測系統(tǒng),DIMSCAN核酸測定系統(tǒng),DNA和基因組檢測;快速即時(shí)的臨床診斷;食品和環(huán)境檢測;**檢測
圖6 培養(yǎng)板單孔細(xì)胞熒光預(yù)覽縮略圖
4)其他用途 
**測定(**、病毒、**、病原體)和蛋白質(zhì)組檢測;核酸,DNA和基因組檢測;快速即時(shí)的臨床診斷;食品和環(huán)境檢測;**檢測

4、參數(shù) 
倒置熒光顯微鏡:Microscope: Olympus IX50 inverted microscope

步進(jìn)電動平移臺:Prior Pro Scan, 步進(jìn)電機(jī)200步/轉(zhuǎn); 250微步/整步; S曲線加速
(stepper motors 200 steps/revolution; 250 microsteps/full step; S-curve acceleration)

CCD相機(jī): QImaging Retiga Exi
CCD分辨率:1024/768
擁有內(nèi)部冷卻功能 
像素混合模式:1-4
(CCD resolution 1024/768;internal cooling;binning modes available: 1-4)

配置英特爾酷睿2雙核處理器以上的計(jì)算機(jī) 
基本配置: 
奔騰III 800 MHz 或者更高;256 MB RAM或更高; 
集成了IEEE1394火線接口的PCI卡 
(Pentium III 800 MHz, 256 MB RAM; IEEE1394 FireWire interface PCI card)

操作系統(tǒng)(Operating System: Windows 2000,XP


DIMSCAN自動熒光數(shù)字圖像顯微處理系統(tǒng),DIMSCAN細(xì)胞毒性測定系統(tǒng),DIMSCAN活細(xì)胞的相對數(shù)測定系統(tǒng),DIMSCAN**測定(**、病毒、**、病原體)系統(tǒng),DIMSCAN蛋白質(zhì)組檢測系統(tǒng),DIMSCAN核酸測定系統(tǒng),DNA和基因組檢測;快速即時(shí)的臨床診斷;食品和環(huán)境檢測;**檢測
圖7 細(xì)胞培養(yǎng)板掃描過程圖示

5、使用方法 
(1)DIMSCAN用戶界面組成 
程序窗口的主要部分包括:
控制面板 
——完整的板圖片 
——支持通過選定孔導(dǎo)航階段 
——任意孔掃描選擇 
——掃描完成該孔含有重建圖像 
——嚴(yán)重掃描孔重新掃描已啟用

現(xiàn)場攝像頭控制 
——實(shí)時(shí)圖像流 
——相機(jī)控制,包括閾值 
縮略圖 
——個(gè)別培養(yǎng)孔掃描高分辨率圖像重建
DIMSCAN自動熒光數(shù)字圖像顯微處理系統(tǒng),DIMSCAN細(xì)胞毒性測定系統(tǒng),DIMSCAN活細(xì)胞的相對數(shù)測定系統(tǒng),DIMSCAN**測定(**、病毒、**、病原體)系統(tǒng),DIMSCAN蛋白質(zhì)組檢測系統(tǒng),DIMSCAN核酸測定系統(tǒng),DNA和基因組檢測;快速即時(shí)的臨床診斷;食品和環(huán)境檢測;**檢測
圖8 DIMSCAN 半自動熒光數(shù)字圖像顯微處理系統(tǒng)觀察界面


(2)主要操作流程 
根據(jù)具體說明書進(jìn)行。

6、系統(tǒng)組成部分 
倒置熒光顯微鏡,步進(jìn)電機(jī)掃描器,掃描控制器,CCD像機(jī)(軟件Qimaging Microimager Ⅱ),微型計(jì)算機(jī)。
DIMSCAN自動熒光數(shù)字圖像顯微處理系統(tǒng),DIMSCAN細(xì)胞毒性測定系統(tǒng),DIMSCAN活細(xì)胞的相對數(shù)測定系統(tǒng),DIMSCAN**測定(**、病毒、**、病原體)系統(tǒng),DIMSCAN蛋白質(zhì)組檢測系統(tǒng),DIMSCAN核酸測定系統(tǒng),DNA和基因組檢測;快速即時(shí)的臨床診斷;食品和環(huán)境檢測;**檢測
圖9 DIMSCAN觀測系統(tǒng)的組成模塊


參考文獻(xiàn)

  1. Proffitt RT, Tran JV, Reynolds CP. A fluorescence digital image microscopy system for quantitating relative cell numbers in tissue culture plates. Cytometry 24:204-213. 1996

  2. Krejsa J, Frgala F, Alfaro P, Reynolds CP. DIMSCAN 3.0, A New Generation of Fluorescence Digital Image Microscopy System for Measuring Cytotoxicity in Microplates. 2002 AACR Annual Meeting (poster). 2002

  3. Ondrej Kalous, Julie Watanabe, Kun Jung Lee, A. Linn Murphree, C. Patrick Reynolds. Use Of A Novel Microplate Fluorescence Cytotoxicity Assay (Dimscan-384) To Evaluate Combinations Of Cytotoxic Agents In A Panel Of Retinoblastoma Cell Lines. 2003 AACR Annual Meeting. 2003

  4. Ondrej Kalous, Jiri Krejsa, Tomas Frgala, C. Patrick Reynolds. The DIMSCAN cytotoxicity assay, unlike the MTT assay, identifies syngergistic combinations of anticancer agents.. 2004 AACR Annual Meeting. 2004

  5. Keshelava, N., Frgala, T., Krejsa, J., Kalous, O., and Reynolds, C. P.. DIMSCAN: a microcomputer fluorescence-based cytotoxicity assay for preclinical testing of combination chemotherapy.. Methods Mol.Med., 110: 139-153. 2005

  6. Frgala T, Kalous O, Proffitt RT, Reynolds CP. A fluorescence microplate cytotoxicity assay with a 4-log dynamic range that identifies synergistic drug combinations.. Mol Cancer Ther, 6(3):886-97. 2007

ABSTRCT FOR DIMSCAN

DIMSCAN is a semi-automatic digital image microscopy system for quantitating relative cell numbers in tissue culture plates. Cytotoxicity assays measured by DIMSCAN using fluorescein diacetate (FDA), a dye accumulating selectively in viable cells, can achieve a 4 log dynamic range at 4 to 7 days and correlate with colony forming assays. The system consists of an inverted fluorescence microscope, stepper motor scanning stage, the stage controller, CCD camera and a microcomputer running the main application, which controls stage movement and processes CCD camera images. Relative numbers of viable cells are determined by evaluating FDA fluorescence intensity, with background fluorescence eliminated by digital thresholding and a quenching of florescence in non-viable cells with the vital stain Eosin Y. The current system uses Olympus IX50 inverted florescence microscope, a Prior motorized stage, a Qimaging Microimager II camera, and a Pentium III computer running Windows 2000. Rectangular plates can be employed with number of wells ranging from 6 to 96 wells per plate. Average scan time for a 96 well plate is 6 minutes.

apers on DIMSCAN development

Authors

Paper name

Published in

Year

Proffitt RT, Tran JV, Reynolds CP

A fluorescence digital image microscopy system for quantitating relative cell numbers in tissue culture plates

Cytometry24:204-213

1996

Krejsa J, Frgala F, Alfaro P, Reynolds CP

DIMSCAN 3.0, A New Generation of Fluorescence Digital Image Microscopy System for Measuring Cytotoxicity in Microplates

2002 AACR Annual Meeting (poster)

2002

Ondrej Kalous, Julie Watanabe, Kun Jung Lee, A. Linn Murphree, C. Patrick Reynolds

Use Of A Novel Microplate Fluorescence Cytotoxicity Assay (Dimscan-384) To Evaluate Combinations Of Cytotoxic Agents In A Panel Of Retinoblastoma Cell Lines

2003 AACR Annual Meeting

2003

Ondrej Kalous, Jiri Krejsa, Tomas Frgala, C. Patrick Reynolds

The DIMSCAN cytotoxicity assay, unlike the MTT assay, identifies syngergistic combinations of anticancer agents.

2004 AACR Annual Meeting

2004

Keshelava, N., Frgala, T., Krejsa, J., Kalous, O., and Reynolds, C. P.

DIMSCAN: a microcomputer fluorescence-based cytotoxicity assay for preclinical testing of combination chemotherapy.

Methods Mol.Med., 110: 139-153

2005

Frgala T, Kalous O, Proffitt RT, Reynolds CP

A fluorescence microplate cytotoxicity assay with a 4-log dynamic range that identifies synergistic drug combinations.

Mol Cancer Ther,6(3):886-97

2007


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Papers using DIMSCAN:

Authors

Paper name

Published in

Year

Reynolds CP, Schindler P, Jones D, Gentile J, Proffitt R, Einhorn P

Comparison of 13-cis- retinoic acid to trans-retinoic acid using human neuroblastoma cell lines

Advances in Neuroblastoma Research 4, Evans A, Biedler JL, Brodeur G, D'Angio GJ, Nakagawara A (eds.), New York: John Wiley & Sons, 237-244

1994

Keshelava N, Seeger RC, Reynolds CP

Drug resistance phenotype of neuroblastoma cell lines established at diagnosis and at relapse after induction chemotherapy or bone marrow transplantation

European J Cancer33:2002-2006

1997

Anderson CP, Tsai JM, Chan WW, Liu RM, Forman HJ, Reynolds CP

Buthionine sulfoximine (BSO) is cytotoxic via apoptosis and synergistically enhances the activity of melphalan (L-PAM) in human neuroblastoma cell lines

European J Cancer 33:2016-2019

1997

Keshelava N, Seeger RC, Groshen S, Reynolds CP

Drug resistance patterns of human neuroblastoma cell lines derived from patients at different phases of therapy

Cancer Research 58:5396-5405

1998

Anderson CP, Tsai JM, Meek WE, Liu RM, Tang Y, Forman HJ, Reynolds G P

Depletion of Glutathione (GSH) by buthionine sulfoximine (BSO) is cytotoxic for human neuroblastoma cell lines via apoptosis

Experimental Cell Research246:183-192

1999

Maurer BJ, Metelitsa LS, Seeger RC, Cabot MC, Reynolds CP

N-(4-hydroxypheynl)retinamide increases ceramide and reactive oxygen species and induces mixed apoptosis/necrosis in neuroblastoma cell lines

J Natl Cancer Inst 91:1138-1146

1999

Keshelava N, Groshen S, Reynolds CP

Cross-resistance of topoisomerase I and II inhibitors in neuroblastoma cell lines

Cancer Chemotherapy and Pharmacology45:1-8

2000

Chen RL, Reynolds CP, Seeger RC, Cabot MC, Reynolds CP

Neutrophils are cytotoxic and growth inhibiting for neuroblastoma cells with anti-GD2 antibody but without cytotoxicity can be growth stimulating

Clinical Immunol Immunother48:603-612

2000

Maurer BJ, Cabot MC, Reynolds CP

Synergism of N-(4-hydroxyphenyl)retinamide cytotoxicity by modulators of ceramide metabolism in solid tumor cell lines

J Natl Cancer Inst 92:1897-1908

2000

Reynolds CP, Wang Y, Melton LJ, Einhorn PA, Slamon DJ, Maurer BJ

Retinoic-acid resistant neuroblastoma cell lines show altered myc regulation and high sensitivity to fenretinide

Medical Pediatric Oncology35:597-602

2000

Keshelava N, Zuo JJ, Chen P, Waidyaratine SN, Luna MC, Gomer CJ, Triche CJ, Reynolds CP

Loss of p53 function confers high-level multi-drug resistance in neuroblastoma cell lines

Cancer Research 61:6185-6193

2001

Anderson CP, Seeger RC, Satake N, Meek WE, Keshelava N, Bailey HH, Monforte-Munoz HL, Reynolds CP

Buthionine sulfoximine and myeloablative concentrations of melphalan overcome resistance in a melphalan-resistant neuroblastoma cell line

J Pediat Hematol Oncol 23:500-505

2001

Metelitsa LS, Gillies SD, Super M, Shimada H, Reynolds CP, Seeger RC

Enhanced expression and activation of Mac-1(CD11b/CD18) by an anti-GD2/GM-CSF fusion protein increases neutrophil antibody dependent cellular cytotoxicity

Blood 99:4166-4173

2002

O'Donnell PH, Guo WX, Reynolds CP, Maurer BJ

N-(4-hydroxyphenyl)retinamide increases ceramide and is cytotoxic to acute lymphoblastic leukemia cell lines, but not to non-malignant lymphocytes

Leukemia 16:902-910

2002

Anderson C, Reynolds CP

Cytotoxicity of buthionine sulfoximine (BSO) and melphalan/BSO in combination for neuroblastoma cell lines derived after myeloablative therapy

Bone Marrow Transplantation30:135-140

2002

Peter J. Houghton, Peter C. Adamson, Susan Blaney, Howard A. Fine, Richard Gorlick, Michelle Haber, Lee Helman, Steve Hirschfeld, Melinda G. Hollingshead, Mark A. Israel, Richard B. Lock, John M. Maris, Glenn Merlino, Wendy Patterson, C. Patrick Reynolds, Kevin Shannon, Alice Yu, John Yu, and Malcolm A. Smith

Testing of New Agents in Childhood Cancer Preclinical Models : Meeting Summary

Clinical Cancer Research8:3646-3657

2002

Bo Yang, Nino Keshelava, Clarke P. Anderson, and C. Patrick Reynolds

Antagonism of Buthionine Sulfoximine Cytotoxicity for Human Neuroblastoma Cell Lines by Hypoxia Is Reversed by the Bioreductive Agent Tirapazamine

Cancer Research 63:1520-1526

2003

Nino Keshelava, Denice Tsao-Wei, and C. Patrick Reynolds

Pyrazoloacridine Is Active in Multidrug-resistant Neuroblastoma Cell Lines with Nonfunctional p531

Clinical Cancer Research9:3492-3502

2003

Grigoryan, R., Keshelava, N., Anderson, C., and Reynolds, C. P.

In vitro testing of chemosensitivity in physiological hypoxia.

Methods Mol.Med., 110: 87-100

2005

Reynolds, C. P. and Maurer, B. J.

Evaluating response to antineoplastic drug combinations in tissue culture models.

Methods Mol.Med., 110: 173-183

2005

Yang, B. and Reynolds, C. P.

Tirapazamine cytotoxicity for neuroblastoma is p53 dependent.

Clin.Cancer Res., 11: 2774-2780

2005

Kang HG, Jenabi JM, Zhang J, Keshelava N, Shimada H, May WA, Tony Ng T, Reynolds CP, Triche TJ, Sorensen PHB

E-cadherin cell-cell adhesion in Ewing tumor cells mediates suppression of anoikis through activation of the ErbB4 tyrosine kinase.

Cancer Research, 67: 3094-3105

2007

Houghton PJ, Morton CL, Tucker C, Gorlick R, Kolb EA, Zhang W, Lock R, Carol H, Reynolds CP, Keshelava N, Maris JM, Courtright J, Keir ST, Friedman HS, Stopford C, Wu J, Smith MA

Stage 1 testing of the proteasome inhibitor bortezomib by the Pediatric Preclinical Testing Program.

Pediatric Blood & Cancer,(In Press).

2007

Keshelava N, Davicioni E, Zesheng Wan Z, Ji L, Sposto R, Triche TJ, Reynolds CP

Inhibition of histone deacetylase (HDAC) 1, a drug target identified by expression profiling, sensitizes multi-drug-resistant neuroblastoma cell lines to cytotoxic agents.

J National Cancer Institute, 99:1107-19

2007

Maris JM, Courtright J, Houghton PJ, Morton CL, Gorlick R, Kolb EA, Lock R, Tajbakhsh M, Reynolds CP, Keir ST, Wu J, Smith MA

Initial testing (Stage 1) of the VEGFR inhibitor AZD2171 by the Pediatric Preclinical Testing Program.

Pediatric Blood & Cancer.,(In Press)

2007

Kang MH, Kang YH, Szymanska B, Wilczynska-Kalak U, Sheard MA, Harned T, Lock RB, Reynolds CP

Activity of vincristine, L-ASP, and dexamethasone against acute lymphoblastic leukemia is enhanced by the BH3-mimetic ABT-737 in vitro and in vivo.

Blood, 110:205-2066

2007

Tajbakhsh M, Houghton PJ, Morton CL, Kolb EA, Maris JM, Keir ST, Wu J, Reynolds CP, Smith MA, Lock RB

Initial testing (Stage 1) of cisplatin by the Pediatric Preclinical Testing Program.

Pediatric Blood & Cancer,(In Press)

2007

Kolb EA, Gorlick R, Houghton PJ, Morton CL, Lock R, Tajbakhsh M, Reynolds CP, Maris JM, Keir ST, Billups CA, Smith MA

Initial testing of dasatinib by the Pediatric Preclinical Testing Program.

Pediatric Blood & Cancer,(In Press)

2007

Reynolds CP, Kang MH, Keshelava N, Mauer BJ

Assessing combinations of cytotoxic agents using leukemia cell lines.

Current Drug Targets,8:765-771

2007


[ Back to Top ]

Clinical Trials Enabled by DIMSCAN Testing:


Authors

Paper name

Published in

Year

Villablanca JG, Avramis V, Khan A, Matthay KK, Ram say NKC, Seeger RC, Reynolds CP

Phase I trial of 13-cis-retinoic acid (cRA) in neuroblastoma patients following bone marrow transplantation (BMT)

J Clinical Oncology13:894-901

1995

Matthay KK, Villablanca JG, Seeger RC, Stram DO, Harris RE, Ramsay NK, Swift P, Shamada H, Black CG, Brodeur GM, Gerbing R, Reynolds CP

Treatment of high risk neuroblastoma with intensive chemotherapy, radiotherapy, autologous bone marrow transplantation, and 13-cis-retinoic acid

New Eng J Med 341:1165-1173

1999

Villablanca JG, Ames MM, Reid JM, Bagniewski P, Krail M, Reynolds CP

Phase I trial of oral [N- (-4-hydroxyphenyl) retinamide] (4-HPR) in children with resistant/recurrent solid tumors: A Children's Cancer Group Study (CCG 09709)

Proc Amer Soc Clin Oncol21:398a

J Clinical Oncology24:3423-3430

2002


2006

Anderson CP, Robert Seeger RC, Bailey H, Reynolds CP

Pilot of buthionine sulfoximine (BSO) combined with non-myeloablative melphalan (L-PAM) against refractory neuroblastoma (NB)

Proc Amer Soc Clin Oncol21 :298a

2002

New Approaches to Neuroblastoma Therapy

N2002-01: A Phase I Study of High-Dose Pyrazoloacridine (PZA) (NSC 366140) Supported with Autologous Hematopoietic Stem Cell Rescue in Children with Recurrent or Resistant Neuroblastoma.

Closed

?

Children's Oncology Group

ANBL0321: A Phase II Study of Fenretinide in Children with Recurrent/Resistant High Risk Neuroblastoma.

Ongoing

?

New Approaches to Neuroblastoma Therapy

N99-02: A Phase I trial of BSO + L-PAM and Stem Cell Support.

Ongoing

?

Norris Cancer Center

A Phase II trial of Fenretinide in Recurrent Ovarian Cancer.

Proc Amer Soc Clin Oncol23:461

Proc Amer Soc Clin Oncol25: Abst 5555

2004


2007

California Cancer Consortium

A Phase II trial of Fenretinide in Prostate Cancer.

Ongoing

?

California Cancer Consortium

A Phase I trial of Intravenous Fenretinide in Hematological Malignancies.

Ongoing

?

California Cancer Consortium

A phase I trial of intravenous fenretinide in solid tumors

Ongoing

?

California Cancer Consortium

A phase II trial of fenretinide in asymptomatic rising PSA prostate cancer

Completed

?

New Approaches to Neuroblastoma Therapy

N2004-04: A Phase I Study of Fenretinide Lym-X-SorbTM (LXS) Oral Powder in Patients with Recurrent or Resistant Neuroblastoma (IND # 68,254)

Ongoing

?

Therapeutic Advances in Childhood Leukemia

TACL 2005-001: A phase I/II trial of ABT-751 combined with dexamethasone, PEG-asparaginase, and doxorubicin in relapsed acute lymphoblastic leukemia (ALL)

Ongoing

?

Therapeutic Advances in Childhood Leukemia

TACL 2005-003: Bortezomib with chemotherapy for relapsed childhood ALL.

Ongoing

?

Therapeutic Advances in Childhood Leukemia

TACL 2006-001: IV Fenretinide in Relapsed ALL, AML or NHL.

Ongoing

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