PrimeCoat series The ExCellness PrimeCoat series is designed specifically to provide a biomimetic cell culture environment that improves cell characteristics and phenotype in laboratory application

產(chǎn)品描述

PrimeCoat series

The ExCellness PrimeCoat series is designed specifically to provide a biomimetic cell culture environment that improves cell characteristics and phenotype in laboratory applications.

Key features

PrimeCoat elastic substrates are easy to use for cell culture and subsequent analysis.
PrimeCoat elastic substrates are available with 6 degrees of softness within the elasticity range of body tissues: 2, 5, 10, 15, 30 and 100 kPa.
PrimeCoat elastic substrates are available in 4 standard formats: 100 mm diameter dishes, 40 mm diameter dishes, 24-well plates and 20x20 mm cover slips.
PrimeCoat elastic substrates require to be coated by the end user to promote cell adhesion.
PrimeCoat elastic substrates are transparent. Cells can be visualized with standard transmission light microscopes (e.g., Phase contrast, DIC).
PrimeCoat elastic substrates are compatible with most standard molecular or cellular techniques: immunofluorescence, immunohistochemistry, protein analysis (e.g., Western blotting, and RNA/DNA extraction).

Biomimetic cell culture substrates at reach
PrimeCoat series combines simplicity and accessibility:

simplicity of cell handling
straightforward compatibility with cell analysis tools
accessible prices

產(chǎn)品：

Dishes

2 kPa culture surface 100 mm ? /35 mm ?
5 kPa culture surface 100 mm ? /35 mm ?
10 kPa culture surface 100 mm ? /35 mm ?
15 kPa culture surface 100 mm ? /35 mm ?
30 kPa culture surface 100 mm ? /35 mm ?
100 kPa culture surface 100 mm ? /35 mm ?

Multi-well plates

2 kPa culture surface 24-well plate
5 kPa culture surface 24-well plate
10 kPa culture surface 24-well plate
15 kPa culture surface 24-well plate
30 kPa culture surface 24-well plate
100 kPa culture surface 24-well plate

Cover slips

2 kPa culture surface 20x20 mm c.slip
5 kPa culture surface 20x20 mm c.slip
10 kPa culture surface 20x20 mm c.slip
15 kPa culture surface 20x20 mm c.slip
30 kPa culture surface 20x20 mm c.slip
100 kPa culture surface 20x20 mm c.slip

ExCellness in peer reviewed publications

In the following peer-reviewed publications, ExCellness biomimetic cell culture devices have been used or cited:

NP Talele et al. Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells. Stem Cell Reports. 2015 Jun 9;4(6) : 1016-30.

V Sarrazy et al. Integrins avb5 and avb3 promote latent TGF-beta 1 activation by human cardiac fibroblast contraction. Cardiovasc Res (2014) 102 (3)
VF Achterberg et al. The nano-scale mechanical properties of the extracellular matrix regulate dermal fibroblast function.J Invest Dermatol. 2014 Jul;134(7):1862-72.
JA Cadby et al. Differences between the Cell Populations from the Peritenon and the Tendon Core with Regard to Their Potential Implication in Tendon Repair. 2014. PLoS ONE 9(3): e92474.
JA Cadby. Can we improve tendon healing in the horse? A multi-angle study of a multi-facet problem. ISBN: 978-90-5335-715-6
A Vashist et al. Recent advances in hydrogel based drug delivery systems for the human body. J. Mater. Chem. B, 2014,2, 147-166
EP van der Veer et al. The RNA-Binding Protein Quaking is a Critical Regulator of Vascular Smooth Muscle Cell Phenotype. Circ Res. 2013 Oct 12;113(9):1065-75.
A De Boeck et al. Differential secretome analysis of cancer-associated fibrobroblasts and bone marrow-derived precursors toidentify microenvironmental regulators of colon cancerprogression. Proteomics 2013,13,379-388
C Godbout et al. The Mechanical Environment Modulates Intracellular Calcium Oscillation Activities of Myofibroblasts.PLoS One. 2013; 8(5): e64560.
S Constant et al. Colon Cancer: Current Treatments and Preclinical Models for the Discovery and Development of New Therapies. Drug discovery; Editor Hany A. El-Shemy. ISBN 978-953-51-0906-8.
JL Balestrini et al. The mechanical memory of lung myofibroblasts. Integr. Biol., 2012,4, 410-421.
Stem Cells and Cancer Stem Cells, Volume 8. Therapeutic Applications in Disease and Injury. Editors M.A. Hayat. ISBN 978-94-007-4797-5

A Skardal et al. Bioprinted amniotic fluid-derived stem cells accelerate healing of large skin wounds. Stem Cells Transl Med. 2012 Nov;1(11):792-802. doi: 10.5966/sctm.2012-0088. Epub 2012 Oct 29.
A Skardal et al. Substrate elasticity controls cell proliferation, surface marker expression and motile phenotype in amniotic fluid-derived stem cells. J Mech Behav Biomed Mater. 2013 January; 17: 307-316.
BJ Crider et al. Myocardin-Related Transcription Factors A and B Are Key Regulators of TGF-b1-Induced Fibroblast to Myofibroblast Differentiation. Journal of Investigative Dermatology (2011) 131, 2378-2385
L Follonier Castella et al. A new lock-step mechanism of matrix remodelling based on subcellular contractile events. Journal of Cell Science 123, 1751-1760

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代理excellness 5 kPa culture surface 20x20 mm c.slip

PrimeCoat series The ExCellness PrimeCoat series is designed specifically to provide a biomimetic cell culture environment that improves cell characteristics and phenotype in laboratory application

Multi-well plates

Cover slips