美國(guó)Tissue growth專(zhuān)注組織工程、生物材料和再生醫(yī)學(xué)等領(lǐng)域用于促進(jìn)組織細(xì)胞增長(zhǎng)和刺激組織發(fā)育的生物反應(yīng)器，提供：
ProductsOsteoGen: Perfusion Bioreactor Systems
BISS's OsteoGen bioreactors impart perfusion through cell seeded cylindrical scaffolds. Applications include investigating cell function, modulating the growth and development of engineered tissues, or acting as a test bed for drug and regenerative medicine technologies. Researchers are currently utilizing these systems in a wide range of research areas including:
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Bone stem cell phenotype research
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Mineral deposition of marrowstromal cells
1O OsteoGen
The 1O system has been designed as a single sample bioreactor system that is able to support a wide range of cell and tissue growth experiments. This chamber can accommodate a single sample with a maximum size of 10 mm diameter x 10 mm thick. Optional features such as: transducers, non-contact micrometers, pressure sensors, etc., and/or modules to customize the instrument to specific needs can be added to accommodate the research application.
12O OsteoGen
The 12O system has been designed as a single sample multi-chamber bioreactor system that is able to support a wide range of cell and tissue growth experiments via user defined stimulation protocols. This chamber can accommodate a single sample with a maximum size of 10 mm diameter x 10 mm thick. A custom manifold integrates up to 12 chambers with individual flow loops. Optional features such as; transducers, non-contact micrometers, pressure sensors, etc., and/or modules to customize the instrument to specific needs can be added to accommodate the research application.
BONE
Key: S=Standard, A=Available, - = not available
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CHAMBERS
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1O
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12O
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Individual growth chamber
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S
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S
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12 parallel growth chambers
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A
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Max construct size: 6 mm diameter by 10 mm long
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S
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S
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STIMULATION
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Pulsatile fluid flow stimulator
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A
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A
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CONTROLS
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Controller software, computer, and monitor
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Power supplies and cabling
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Mean flow control
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A
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A
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Dynamic flow control
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TISSUE MONITORING
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Non-contact flow sensors
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A
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A
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Catheter pressure transducers
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A
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A
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ACCESSORIES
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Media reservoir
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S
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S
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Incubator
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A
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A
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BENEFITS
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All-autoclavable components
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S
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S
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Excellent sample access
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S
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S
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Medium change without opening chamber via luer fittings
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S
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S
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Increased sterility with fast and clean sample change and access
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S
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S
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Lightweight design for easy handling
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S
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S
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Easily cleaned surface finishes
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S
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S
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Compact design for fit in standard incubators
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S
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S
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100% satisfaction guaranteed warranty
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S
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Publications
PublicationsBISS TGT Bioreactor Systems in Current Literature
Patents
Instrumented bioreactor with material property measurment capability and process-based adjustment for conditioning tissue engineered medical products.US pat no 7410792. August 12, 2008
Bioreactor with plurality of chambers for conditioning intravascular tissue engineered medical products. US pat no 7348175. March 25, 2008
Cell seeding module including an apparatus and method for seeding cells on a sample or specimen. US pat no 8173420. May 8, 2012.
Peer Reviewed Publications
Angelidis IK, Thorfinn J, Connolly ID, Lindsey D, Pham HM, Chang J. Tissue Engineering of Flexor Tendons: The Effect of a Tissue Bioreactor on Adipoderived Stem cell-Seeded and Fibroblast-Seeded Tendon Constructs. J Hand Surg Am. 2010 Sep; 35(9): 1466-72.
Woon Cy, Pridgen BC, Kraus A, Bari S, Pham H, Chang J. Optimization of Human Tendon Tissue Engineering: Peracetic Acid Oxidation for Enhanced Reseeding of Acellularized Intrasynovial Tendon. Plast Reconstrc Surg. 2011 March; 127(3):1107-17
Woon Cy, Kraus A, Raghavan SS, Pridgen BC, Megerle K, Pham H, Chang J. Three-Dimensional-Construct Bioreactor Conditioning in Human Tendon Tissue Engineering. Tissue Eng Part A. 2011 July 1: Epublished ahead of print
Tran SC, Cooley AJ, Elder SH. Effects of a Mechanical Stimulation Bioreactor on Tissue Engineered, Scaffold-Free Cartilage. Biotechnology and Bioengineering. 2011; 108:1421-1429.Saber S, Zhang AY, Ki SH, Lindsey DP, Smith RL, Riboh J, Pham H, Chang J. Flexor Tendon Tissue Engineering: Bioreactor Cyclic Strain Increases Construct Strength. Tissue Engineering A. 2010 Jun 16(6): 2085-90.
Fischer LJ, McIlhenny S, Tulenko T, Golesorkhi N, Zhang P, Larson R, Lombardi J, Shapiro I, DiMuzio P. Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force. Journal of Surgical Research. 2009 March; 152 (1):157-166. PubMed PMID 19883577.
Harris LJ, Abdollahi H, Zhang P, McIlhenny S, Tulenko T, DiMuzio PJ. Differentiation of Adult Stem Cells into Smooth Muscle for Vascular Tissue Engineering. Journal of Surgical Research. Article in Press [Epub ahead of print] September 4, 2009. PubMed PMID 19959190.
McIlhenny S, Hager ES, Grabo DJ, DiMatteo C, Shapiro IM, Tulenko T, DiMuzio PJ. Linear Shear Conditioning Improves Vascular Graft Retention of Adipose-Derived Stem Cells by Upregulation of a5?1 Integrin. Tissue Engineering Part A. 2010 Jan; 16(1): 245-255.
Klein TJ, Malda J, Sah RL, Hutmacher DW, Tissue Engineering of Articular Cartilage with Biomimetic Zones. Tissue Engineering Part B. 2009 Feb 9 PubMed PMID 19203206.
Cartmell SH, Porter BD, Garcia AJ, Guldberg RE, Effects of Medium Perfusion Rate on Cell-Seeded Three-Dimensional Bone Constructs In Vitro. Tissue Eng. 2003 Dec;9(6):1197-203.
McClure MJ, Sell SA, Ayres CE, Simpson DG, Bowlin GL. Electrospinning-aligned and random polydioxanon-polycaprolactone-silk-fibroin-blended scaffolds: geometry for a vascular matrix. Biomedical Materials. 2009; 4(5). PubMed PMID 19815970.
Mohan N, Nair PD, Tabata Y. Growth factor-mediated effects on chondrogenic differentiation of mesenchymal stem cells in 3D semi-IPN poly(vinylalcohol)-poly(caprolactone) scaffolds. J Biomed Mater Res A. 2010 Feb 2. [Epub ahead of print] PubMed PMID: 20128001.
Porter BD, Lin AS, Peister A, Hutmacher D, Guldberg RE, Noninvasive image analysis of 3D construct mineralization in a perfusion bioreactor. Biomaterials.2007 May; 28(15):2525-33. Epub 2007 Jan 26.
Sell SA, McClure MJ, Barnes CP, Knapp DC, Walpoth BH, Simpson DG, Bowlin GL. Electrospun polydioxanone-elastin blends: potential for bioresorbably vascular grafts. Biomedical Materials. 2006; 1(2).PubMed PMID 18460759.
Smith MJ, McClure MJ, Sell SA, Barnes CP, Walpoth BH, Simpson DG, Bowlin GL. Suture-reinforced electrospun polydioxanone-elastin small-diameter tubes for use in vascular tissue engineering: A feasibility study. Acta Biomaterialia. 2008 Jan;4(1):58-66. PMID 17897890.
Voge CM, Kariolis M, MacDonald RA, Stegemann JP. Directional conductivity in SWNT-collagen-fibrin composite biomaterials through strain-induced matrix alignment. J Biomed Mater Res A. 2008 Jul;86(1):269-77. PubMed PMID: 18428799.
Michael J. McClure, Scott A. Sell, David G. Simpson, Beat H. Walpoth, Gary L. Bowlin. A three-layered electrospun matrix to mimic native arterial architecture using polycaprolactone, elastin, and collagen: A preliminary study. Acta Biomaterialia. Vol. 6, Issue 7, July 2010, Pages 2422-2433.
Dr. Jan Hansmann, Florian Groeber, Alexander Kahlig, Claudia Kleinhans, Heike Walles. Bioreactors in tissue engineering--principles, applications and commercial constraints. Biotechnology Journal. Vol. 8, Issue 2, 2013.
Johan Thorfinn, I.K. Angelidis, L. Gigliello, H.M. Pham, D. Lindsey, J. Chang. Bioreactor optimization of tissue engineered rabbit flexor tendons in vivo. The Journal of Hands Surgery. (Eur Vol.) Feb. 2012 vol. 37 no. 2 pages 109-114.
Presentations
Christopher M. Voge, Mihalis Kariolis, Rebecca A. MacDonald, Jan P. Stegemann, Directional Conductivity in Protein-Nanotube Biomaterials through Strain-Induced Matrix Alignment. 8th World Biomaterials Congress. Amsterdam, Netherlands, June 2008.
S Saber. Stanford University Medical Center, Department of Plastic Surgery, Flexor Tendon Tissue Engineering: Cyclic Strain Increases Construct Strength and Tendon Architecture. Plastic Surgery Research Council. Springfield, Illinois, May 2008. Also presented at the California Society of Plastic Surgeons, Dana Point, California, June 2008.
BD Porter, A Peister, D Hutmacher, RE Guldberg, Dynamic Culture Conditions Modulate Mineralization Matrix Deposition, Growth Rate, and Particle Size Within Large 3-D Constructs. Transactions of the 2006 Summer Bioengineering Conference, Amelia Island, Florida, June 2006.
BD Porter, A Peister, D Hutmacher, RE Guldberg, In Vitro Perfusion Accelerates the Rate of Mineralized Matrix Formation Within 3-D Constructs by Increasing both the Number and Size of Mineralization Sites. Transactions of the 52nd Annual Orthopaedic Research Society, Chicago, Illinois, March 2006.
BD Porter, Roger Zauel, D Hutmacher, RE Guldberg, D Fyhrie, Perfusion Significantly Increases Mineral Production Inside 3-D PCL Composite Scaffolds.Regenerate International Conference and Exposition, Atlanta, Georgia, June 2005. Also presented at the American Society for Mechanical Engineering Summer Bioengineering Meeting, Vail, Colorado, June 2005. Also presented at Transactions of the 51st Annual Orthopaedic Research Society Meeting, Washington, D.C., February 2005.
Posters
S.E.McIlhenny, D.J.Grabo, N.A. Tarola, P.Zhang, I.M.Shapiro, T.N.Tulenko, and P.J.DiMuzio, Shear Conditioning of Adipose-Derived Stem Cells Increases Retention on Decellularized Vein Grafts. Biomedical Engineering Society Meeting, Los Angeles, California, September 2007.
Whitlock, Patrick, Knutson, James, Smith, Thomas L., Van Dyke Mark E., Shilt, Jeffrey S., Koman, L. Andrew, Poehling, Gary G., Effects of Mechanical Stimulation on a Cell-Seeded Scaffold Developed for Tendon and Ligament Regeneration. Transactions of the 6th Combined Meeting of the Orthopaedic Research Society, Honolulu, Hawaii, October 2007. Also presented at the Transactions of the 54th Annual Orthopaedic Research Society Meeting, San Francisco, California, March 2008.
Mechanical Stimulation in the Literature
Reviews
Barrilleaux, B., et al. 2006. Tissue Engineering. "Review: of Ex Vivo Engineering of Living Tissues with Adult Stem Cells." Oct 1 (on line publishing).
Bilodeau, K. and Mantovani, D. 2006. Tissue Engineering. "Bioreactors for tissue engineering focus on mechanical constraints, A comparative review." Aug: 12 (8) 2367-83.
Ratner, B., et al. 1996. Biomaterials Science: An Introduction to Materials in Medicine. Academic Press. San Diego, CA.
Wendt, D., et. al. . 2006. Biorheology. "Uniform tissues engineered by seeding and culturing cells in 3D scaffolds under perfusion at defined oxygen tensions." 43 (3-4): 418-488.
Mcllhenny, S., et al. 2009. Tissue Engineering. "Linear Shear Conditioning Inproves Vascular Graft Retention of Adipose-Derived Stem Cells by Upregulation." Sept. 21 (15).
Juliane Rauh, Falk Milan, Klaus-Peter Gunther, and Maik Stiehler. Tissue Engineering. "Bioreactor Systems for Bone Tissue Engineering." August 2011, 17(4): 263-280.
Bone
Braccini, A. et al. 2005. Stem Cells. "Three-dimensional perfusion culture of human bone marrow cells and generation of osteoinductive grafts." Sep 23 (8): 1066-72.
Shawn Pl Grogan, Sujata Sovani, Chantal Pauli, Jianfen Chen, Andreas Hartmann, Clifford W. Colwell Jr., Marin K. Lotz, and Darryl D. D'Lima. "Effects of Perfusion and Dynamic Loading on Human Neocartilage Formation of Alginate Hydrogels." Tissue Engineering Part A. September 2012, 18(17-18): 1784-1792.
Vascular
Bouhout S, Perron E, Gauvin R, Bernard G, Ouellet G, Cattan V, Bolduc S. "InVitro Reconstruction of an Autologous, Watertight, and Resistant Vesical Equivalent." Tissue Eng Part A. 2010 Feb 11. [Epub ahead of print] PubMed PMID:20014996.
Shinoka, T. 2002. Artificial Organs. "Tissue Engineered Heat Valves: Autologous Cell Seeding on Biodegradable Polymer Scaffold." 26(5): 402-406.
Yow, K.H., et al. 2006. British Journal of Surgery. "Tissue engineering of vascular conduits." 93(6): 652-661.
Hao-Fan Peng, Jin Yu Liu, Stelios T. Andreadis, and Daniel D. Swartz. "Hair Follicle-Derived Smooth Muscle Cells and Small Intestinal Submucosa for Engineering Mechanically Robust and Vasoreactive Vascular Media." Tissue Engineering Part A. April 2011, 17(7-8): 981-990.
Stem Cell
Willenberg, B.J., et al. 2006. Journal of Biomaterials Res A. "Self-assembled copper-capillary alginate gel scaffolds with oligochitosan support embryonic stem cell growth." 79(2): 440-50.
M.J. Moreno, A. Ajji, D. Mohebbi-Kalhori, M. Rukhlova, A. Jadhizadeh, M.N. Bureau. Journal of Biomaterials Res B. "Development of a compliant and cytocompatible micro-fibrous polyethylene terephthalate vascular scaffold." Vol. 97B, Issue 2, pages 201-213, May 2011.
Scaffolds
Scheindler, M., et al. 2006. Cell Biochemistry and Biophysics. Living in three dimensions: 3D nano structured environments for cell culture and regenerative medicine. 45(2):215-27.
Zahir, N. and Weaver, V.M. 2004. Current Opinion in Genetics and Development Death in the third dimension: apoptosis regulation and tissue architecture.. 14(1): 71-80.
Zhang, S., et. al. 2005. Seminars in Cancer Biology. Designer self –assembling peptide nanofiber scaffolds for 3D tissue cell cultures. 15(5): 413-20.
Jones, D., et. al. 2009. A Versatile Approach to Scaffold Design for Bone in Growth Structures. Clinical Engineering, School of Clinical Sciences, University of Liverpool, UK
Drug Development
Andrei, G. 2006. Antiviral Research. Three-dimensional culture models for human viral diseases and antiviral drug development. 71(2-3): 96-107.