CSTRGPKG (replaceable glass) Culture/stim system
The Culture Chamber system with replaceable glass or plastic bottoms uses a low cost but complete cell perfusion chamber (BTRG) made up of achamber (BTRG-CH), gasket (BTRG-GA), cover glass, and bottom clip (BTRG-BC) for either culturing cells after autoclaving the chambers or attaching a glass or plastic 24x30mm cover slide with cells cultured on it. The made up chamber is then transferring them to a microscopy or electrophysiology setup. The chamber has an inflow tube and area for aspirating solution. We have used the experience from the BT-xx tissue chambers to develop a compact chamber that has laminar flow over a range of flow rates (up to 2ml/min). The Stimulation/thermistor assembly (STIM-AT) presses into the Chamber holder (HLDRG). The unique design of the stimulation electodes is such that they clip into a small recess cut along the front and rear lower edges of the chamber. They can be rebent to their original shape if they are accidentally damaged. The aspirator is postioned with the FL-1 Miniature postioner which also attaches to theHLDRG. In a typical setup with temperature control the HPRE2 Pre-heater is used for perfusing the chamber. The MPRE8 8 channel pre-heater can be used to superfuse a region of the chamber. These can both be controlled by theTC2BIP Temperature Controller. If the TC2BIP is equiped with the third channel (TC2-Ch3) the temperature in the chamber can be monitored with the thermistor built into the Stimulation/thermistor assembly. The Chamber holder fits into the Microscope stage adapter. These are currently available for the Nikon (MSA-NIK)-10cm round insert, Nikon (MSA-TS100), Olympus (MSA-OL11)-11cm round insert) & Leica DMIL (MSA-LEI/DM). Please call to check on the availability of others.
CSTPKG (fixed glass) Culture/stim system
The Culture Chamber system uses a low cost but complete cell perfusion chamber (BT-CS) for culturing cells and then transferring them to a microscopy or electrophysiology setup. The chamber has an inflow tube and area for aspirating solution. We have used the experience from the BT-xx tissue chambers to develop a compact chamber that has laminar flow over a range of flow rates. The Stimulation/thermistor assembly (STIM-AT)presses into the Chamber holder (HLD-CS). The unique design of the stimulation electodes is such that they clip into a small recess cut along the front and rear lower edges of the chamber. They can be rebent to their original shape if they are accidentally damaged. The aspirator is postioned with the FL-1 Miniature postioner which also attaches to the HLD-CS. In a typical setup with temperature control the HPRE2 Pre-heater is used for perfusing the chamber. The MPRE8 8 channel pre-heater can be used to superfuse a region of the chamber. These can both be controlled by theTC2BIP Temperature Controller. If the TC2BIP is equiped with the third channel (TC2-Ch3) the temperature in the chamber can be monitored with the thermistor built into the Stimulation/thermistor assembly. The Chamber holder fits into the Microscope stage adapter. These are currently available for the Nikon (MSA-NIK)-10cm round insert, Nikon (MSA-TS100), Olympus (MSA-OL11)-11cm round insert) & Leica DMIL (MSA-LEI/DM). Please call to check on the availability of others.