BYOSENS LYTE96**臺便攜式無標(biāo)記酶標(biāo)儀（便攜微孔板檢測器）
lyte96無標(biāo)記便攜生物傳感器系統(tǒng)介紹
lyte96無標(biāo)記便攜生物傳感器是基于康寧Epic系統(tǒng)設(shè)計的，可進(jìn)行一系列細(xì)胞內(nèi)試驗的96孔微孔板讀出設(shè)備。lyte96將無線連接和集成電池結(jié)合放置到一個緊湊的結(jié)構(gòu)中，使得它方便移動和易于整合進(jìn)液體處理系統(tǒng)。主要是對系列廣泛的生物反應(yīng)進(jìn)行檢測，如信號轉(zhuǎn)導(dǎo)、細(xì)胞凋亡、細(xì)胞**，貼壁、增殖和擴(kuò)散等。
lyte96無標(biāo)記便攜生物傳感器的工作原理是基于折射波導(dǎo)光柵光學(xué)生物傳感器。傳感器結(jié)構(gòu)由一個三層系統(tǒng)：玻璃基板、薄膜光波導(dǎo)薄膜與光柵結(jié)構(gòu)，和細(xì)胞/生物分子層。當(dāng)寬譜帶光照射時，生物傳感器反映光的特定波長是接近傳感器表面折射率的靈敏函數(shù)。通過 Epic系統(tǒng)測量細(xì)胞內(nèi)的粘合物事件或細(xì)胞內(nèi)蛋白質(zhì)運(yùn)動引起反射光的波長偏移。形成一系列波長偏移、波長、強(qiáng)度、時間之間的函數(shù)來進(jìn)行分析。
lyte96無標(biāo)記便攜生物傳感器的優(yōu)勢：
移動性： lyte96**設(shè)計之處是給使用者帶來了*大的靈活性。緊湊的結(jié)構(gòu)結(jié)合了無線連接和集成的電池使lyte96方便移動。這使得它對于研究人員和開發(fā)人員來說成為一個**的分析工具。
易用性： lyte96簡化了研發(fā)實驗室中的過程。實驗開始時不需要復(fù)雜的預(yù)置，直觀輔助的軟件保證了高水平的易用性。由于**技術(shù)體系，lyte96幾乎是免費維護(hù)。
數(shù)據(jù)分析：根據(jù)已建立的康寧Epic系統(tǒng)，高敏性的lyte96可進(jìn)行寬光譜的細(xì)胞內(nèi)試驗，從開始試驗到幾天的時間都可以提供實時數(shù)據(jù)以便研究。
圖1. 萊特96無標(biāo)記便攜生物傳感器
圖2.測量原理示意圖
例1：在增殖試驗中，用lyte96實時監(jiān)測細(xì)胞數(shù)量，發(fā)現(xiàn)細(xì)胞數(shù)目和傳感器表面的質(zhì)量是成正比的。微孔板和lyte96放置在加濕的培養(yǎng)箱內(nèi)通過藍(lán)牙無線連接電腦。經(jīng)典增殖試驗中，A431細(xì)胞加入到孔中，記錄37?C的細(xì)胞生長。
例2：動態(tài)質(zhì)量再分配（DMR）的測定
像許多其他的信號檢測，GPCR測定動態(tài)質(zhì)量再分配過程中（DMR）是由lyte96無標(biāo)記傳感器測定的。和A431細(xì)胞緩激肽試驗一樣，這個試驗是在室溫下進(jìn)行。得到的EC50為0.45 nm，這類似于從文獻(xiàn)的結(jié)果
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