BYOSENS LYTE96**臺(tái)便攜式無(wú)標(biāo)記酶標(biāo)儀（便攜微孔板檢測(cè)器）

lyte96無(wú)標(biāo)記便攜生物傳感器系統(tǒng)介紹

lyte96無(wú)標(biāo)記便攜生物傳感器是基于康寧Epic系統(tǒng)設(shè)計(jì)的，可進(jìn)行一系列細(xì)胞內(nèi)試驗(yàn)的96孔微孔板讀出設(shè)備。lyte96將無(wú)線連接和集成電池結(jié)合放置到一個(gè)緊湊的結(jié)構(gòu)中，使得它方便移動(dòng)和易于整合進(jìn)液體處理系統(tǒng)。主要是對(duì)系列廣泛的生物反應(yīng)進(jìn)行檢測(cè)，如信號(hào)轉(zhuǎn)導(dǎo)、細(xì)胞凋亡、細(xì)胞**，貼壁、增殖和擴(kuò)散等。

lyte96無(wú)標(biāo)記便攜生物傳感器的工作原理是基于折射波導(dǎo)光柵光學(xué)生物傳感器。傳感器結(jié)構(gòu)由一個(gè)三層系統(tǒng)：玻璃基板、薄膜光波導(dǎo)薄膜與光柵結(jié)構(gòu)，和細(xì)胞/生物分子層。當(dāng)寬譜帶光照射時(shí)，生物傳感器反映光的特定波長(zhǎng)是接近傳感器表面折射率的靈敏函數(shù)。通過(guò) Epic系統(tǒng)測(cè)量細(xì)胞內(nèi)的粘合物事件或細(xì)胞內(nèi)蛋白質(zhì)運(yùn)動(dòng)引起反射光的波長(zhǎng)偏移。形成一系列波長(zhǎng)偏移、波長(zhǎng)、強(qiáng)度、時(shí)間之間的函數(shù)來(lái)進(jìn)行分析。

lyte96無(wú)標(biāo)記便攜生物傳感器的優(yōu)勢(shì)：

移動(dòng)性： lyte96**設(shè)計(jì)之處是給使用者帶來(lái)了*大的靈活性。緊湊的結(jié)構(gòu)結(jié)合了無(wú)線連接和集成的電池使lyte96方便移動(dòng)。這使得它對(duì)于研究人員和開(kāi)發(fā)人員來(lái)說(shuō)成為一個(gè)**的分析工具。

易用性： lyte96簡(jiǎn)化了研發(fā)實(shí)驗(yàn)室中的過(guò)程。實(shí)驗(yàn)開(kāi)始時(shí)不需要復(fù)雜的預(yù)置，直觀輔助的軟件保證了高水平的易用性。由于**技術(shù)體系，lyte96幾乎是免費(fèi)維護(hù)。

數(shù)據(jù)分析：根據(jù)已建立的康寧Epic系統(tǒng)，高敏性的lyte96可進(jìn)行寬光譜的細(xì)胞內(nèi)試驗(yàn)，從開(kāi)始試驗(yàn)到幾天的時(shí)間都可以提供實(shí)時(shí)數(shù)據(jù)以便研究。 

圖1. 萊特96無(wú)標(biāo)記便攜生物傳感器

圖2.測(cè)量原理示意圖

例1：在增殖試驗(yàn)中，用lyte96實(shí)時(shí)監(jiān)測(cè)細(xì)胞數(shù)量，發(fā)現(xiàn)細(xì)胞數(shù)目和傳感器表面的質(zhì)量是成正比的。微孔板和lyte96放置在加濕的培養(yǎng)箱內(nèi)通過(guò)藍(lán)牙無(wú)線連接電腦。經(jīng)典增殖試驗(yàn)中，A431細(xì)胞加入到孔中，記錄37?C的細(xì)胞生長(zhǎng)。

例2：動(dòng)態(tài)質(zhì)量再分配（DMR）的測(cè)定

像許多其他的信號(hào)檢測(cè)，GPCR測(cè)定動(dòng)態(tài)質(zhì)量再分配過(guò)程中（DMR）是由lyte96無(wú)標(biāo)記傳感器測(cè)定的。和A431細(xì)胞緩激肽試驗(yàn)一樣，這個(gè)試驗(yàn)是在室溫下進(jìn)行。得到的EC50為0.45 nm，這類(lèi)似于從文獻(xiàn)的結(jié)果

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