Magnetic 3D Bioprinting
While other spheroid systems can mimic native cellular environments, they take a long time to form and are difficult to handle/retrieve. Magnetic 3D bioprinting addresses these issues by utilizing n3D’s core technology, magnetizing cells with NanoShuttle (NS) to print spheroids. In contrast to magnetic levitation using the Bio-Assembler, in magnetic 3D bioprinting, cells incubated with NS overnight are printed into spheroids by placing a 96-well plate full of magnetized cells atop a drive of magnets.1 The magnets below the well aggregate the cells using mild magnetic forces to form a spheroid at the bottom of the well. Afteronly 15 min to a few hours, the plate of spheroids can be removed from the magnets and cultured long-term. NanoShuttle does not interfere with fluorescence or other biochemical assays, so the spheroid can be assayed in any number of ways.
Viability of PC3 spheroids of various sizes as measured by (left) the Real-time CellTiter-Glo assay (Promega, Madison, WI) and (right) live/dead staining
In magnetizing spheroids, adding and removing solutions is made easy by the use of magnetic forces to hold down spheroids during aspiration, limiting spheroid loss. Spheroids can also be picked up and transferred between vessels using magnetic tools such as the MagPenTM. Magnetic forces can also be used to create co-cultures with fine spatial organization.2
Co-culture of primary human tracheal smooth muscle cells (green) and A549 human alveolar epithelial lung adenocarcinoma cells (red) at varying ratios
Magnetic 3D bioprinting is a rapid and effective tool to print spheroids that are representative of native cellular environments and easy to handle.
Products
|
|
Product
|
Includes
|
Part #
|
Manual
|
Click to Buy!
|
|
96-well Bioprinting Kit
|
-
600-μL Vials of NanoShuttleTM-PL (3)
-
96-Well Spheroid Drive (1)
-
96-Well Holding Drive (1)
-
Cell-Repellent 96-Well Plates (2)
|
011-96WK
|
|
現(xiàn)貨詢價(jià)
|
|
384-well Bioprinting Kit
|
-
600-μL Vials of NanoShuttleTM-PL (2)
-
384-Well Spheroid Drive (1)
-
384-Well Holding Drive (1)
-
Cell-Repellent 384-Well Plates (2)
|
012-384WK
|
|
現(xiàn)貨詢價(jià)
|
*US prices only. Ask your local distributor for prices in your region.
Contact us for additional parts.
References
1. Timm, D. M. et al. A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis. Sci. Rep. 3, 3000 (2013)
2. Tseng, H. et al. Assembly of a three-dimensional multitype bronchiole coculture model using magnetic levitation. Tissue Eng. Part C. Methods 19, 665–75 (2013).