NanoCulture Plate

【NCP(NanoCulture Plate)/NCD(NanoCulture Dish)】

NCP and NCD are culture ware for 3D cell culture. One can get spheroid using these culture ware and general growth media containing a heat inactivated FBS.

Format	Bottom film
	Pattern	Cell adhesiveness
384-well SBS format	Micro-Square(MS) Micro-Honeycomb(MH)	Low Binding High Binding
96-well SBS format
24-well SBS format
35 mm Dish

Please see FAQ’s Start-up for how to select plate type.

Product Name	Cat.#	Qty	Document
NanoCulture Plate MS pattern  Low-Binding, 384-well	NCP-LS384-10	10	Handbook
	NCP-LS384-100	100
NanoCulture Plate MH pattern  Low-Binding, 384-well	NCP-LH384-10	10
	NCP-LH384-100	100
NanoCulture Plate MS pattern  High-Binding, 384-well	NCP-HS384-10	10
	NCP-HS384-100	100
NanoCulture Plate MH pattern  High-Binding, 384-well	NCP-HH384-10	10
	NCP-HH384-100	100

Product Name	Cat.#	Qty	Document
NanoCulture Plate MS pattern  Low-Binding, 96-well	NCP-LS96-2	2	Handbook
	NCP-LS96-10	10
NanoCulture Plate MS pattern  High-Binding, 96-well	NCP-HS96-2	2
	NCP-HS96-10	10
NanoCulture Plate MH pattern  Low-Binding, 96-well	NCP-LH96-2	2
	NCP-LH96-10	10
NanoCulture Plate MH pattern  High-Binding, 96-well	NCP-HH96-2	2
	NCP-HH96-10	10
NanoCulture Plate MS/MH pattern  Low-Binding, 96-well	NCP-LSH96-2	2

Product Name	Cat.#	Qty	Document
NanoCulture Plate MS pattern  Low-Binding, 24-well	NCP-LS24-2	2	Handbook
	NCP-LS24-10	10
NanoCulture Plate MS pattern  High-Binding, 24-well	NCP-HS24-2	2
	NCP-HS24-10	10
NanoCulture Plate MH pattern  Low-Binding、24-well	NCP-LH24-2	2
	NCP-LH24-10	10
NanoCulture Plate MH pattern  High-Binding, 24-well	NCP-HH24-2	2
	NCP-HH24-10	10
NanoCulture Plate MS/MH pattern  Low-Binding, 24-well	NCP-LSH24-2	2

Product Name	Cat.#	Qty	Document
NanoCulture Dish MS pattern  Low-Binding, 35mm	NCD-LS35-5	5	Handbook
NanoCulture Dish MH pattern  Low-Binding, 35mm	NCD-LH35-5	5
NanoCulture Dish MS pattern  High-Binding, 35mm	NCD-HS35-5	5
NanoCulture Dish MH pattern  High-Binding, 35mm	NCD-HH35-5	5

For establishing your cell culture condition for the first time, it is better to use low-binding plates set, micro-square and micro-honeycomb (Cat.# NCP-LSH96-2), and choose the appropriate pattern. 
High-binding plate is suitable for low adherence cell. Please try to use this type of plate when cell has difficulties to attach and form spheroids on the low-binding plate.

For culturing the above cell line, MS pattern plate and M type medium are considered as appropriate culture condition.

How to choose the optimum plate pattern

Two cell lines were cultured with low- and high- binding plates.

In case of the left side cell line, high-binding plate is suitable as the spheroids have difficulties to attach on low-binding plate and grow disproportionately. On the other hand, low-binding plate is suitable for the right side cell line as the cells form monolayer on high-binding plate

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