Moxi GO & Moxi GO II流式細(xì)胞儀—現(xiàn)貨促銷
Product Code: MXG001_MXG002_MXG102
Moxi GO 532nm laser (MXG001)
1 PMT - 561nm/LP filter (e.g. PE, PI) - US Version
Moxi GO 488nm laser (MXG002)
1 PMT - User-swappable 525/45nm (e.g. FITC, GFP) or 561nm/LP (PE,PI) filters
Moxi GO II 488nm laser (MXG102)
2 PMT system 525/45nm (e.g. FITC, GFP) and 561nm/LP (PE, PI)
Overview
Increase the pace of scientific discovery with ORFLO’s next generation Moxi Go? or Moxi Go II? Next Gen Flow Cytometers. With a one (Moxi GO) or two (Moxi GO II) fluorescent channels, swappable filter sets (MXG002 - Moxi GO 488nm), configurable laser the Moxi Go can be designed to meet your labs cell analysis needs. Every assay also delivers simultaneous cell count & volume determination, using single-use flow cell. This eliminates the hassle of traditional flow associated with cleaning, maintenance, clearing of clogs, cross contamination and occasionally replacement of bottles and tubes.
Furthermore the Moxi Go? or Moxi Go II? use very little sample volume, 75ul's allowing you to conserver precious, expensive sample, such as stem cells. Cell concentrations as low as 10,000 cells per ml are possible, which most experiments would enable a little as 5ul's of sample diluted in 70ul's of PBS.
The Moxi Go? or Moxi Go II? can be utilized as an assay development instrument and many powerful cell based assays can easily be optimized and run on the system. These include:
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Cell surface immuno-labeling
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In-Cell Protein Quant
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CRISPR/Transfection Optimization Studies
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Up to 4 plex bead based ELISA's using our new on board MPX Relative Quantitation Ap
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In cell Wester Blots
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Reactive Oxidation Species Experiments
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Calein AM studies
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Phagocytosis Analysis
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Mito Potential Experiements
The Moxi Go? or Moxi Go II? come standard with an ultra-intuitive, plug-and-play interface with free OS updates as long as you own the instrument. No prior flow cytometry experience is required you simply just plug and play.
參數(shù)規(guī)格：
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id
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MXG001_MXG002_MXG102
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Included Accessories
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USB power cord, US style USB power adapter, and Type MF-S cassette pack
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AC Power Type
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110 VAC
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Applications
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Mulitplexed Bead ELISA's|In Cell Westerrns|In Cell Protein Quant|RFP| Gold Standard Cell Count and Viability|Mito Potential|ROS|Phagocytosis
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Average Cell Diameter Range
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3 - 16 microns Type MF-S
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Battery Type
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Rechargeable 3.7 V, 4500 mAh lithium ion
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Cassette Types
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Type MF-S
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Cell Particle Concentration Range
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10,000 - 1,750,000 cells/mL Type MF-S
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Cell Types Tested
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HEK-293|HeLa|PC12|CD3+T|CHO-K1|Cos-7|HepG2|Hybridoma|Jurkat E6-1|K562|MCF7|Mesenchymal SC|Monocyte|Mouse ESC| NIH 3T3| PBMC (cultured)|Red Blood Cells (RBC)|L5178y| C. albicans (Yeast)| S. cerevisiae Vin 13 (Yeast)|S. cerevisiae X5 (Yeast)|Wine Yeast (natural fermentaion)|S.cerevisiae (Baker's Yeast|Safale US-05 Yeast|
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Data Output Formats
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FCS 3.1, screen shots (.bmp), CSV
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Data Storage Capacity
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16Mb and 4GB microSD
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Display Resolution
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800 x 480 color touchscreen
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Excitation Wavelengths
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532 nm (MXG001) or 488nm (MXG002, MXG102)
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In British Units
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7 lbs
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Intended Use Statement
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For Research Use Only. Product is not for use in diagnostic procedures
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Laser Color
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Green(MXG001), Blue (MXG002, MXG102)
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Measurable Dynamic Range
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2 - 25 microns Type MF-S
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Measurement Time
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10 seconds Type MF-S
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MPICell Health Ratio Moxi Viability Index MVI
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Yes (Size histogram only)
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Number of Detection Channels Flow parameters
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MXG001 and MXG002: 3 (1 color, 1 size, 1 transmittance), MX104: 4 (2 color, 1 size, 1 transmittance)
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Number of PMTs
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1
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Optical Detection Range
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MXG001: 561/LP nm (e.g. PE, RFP), MXG002: User-swappable 525/45nm (e.g. FITC, GFP) or 561nm/LP (e.g. PE, RFP), MXG102: 525/45nm (e.g. FITC, GFP) and 561nm/LP (e.g. PE, RFP)
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Particle Size Detection Method
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Impedimetric (Coulter Principle)
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Platform
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Open platform: 561nm/LP (PI, PE, DS Red, Sytox Orange, 7 AAD, Nile Red, Rhodamine Red, Sun Coast Yellow, PE/Cy5), 525/45nm (FITC, GFP, Alexa Fluor 488nm, Calcein)
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Pre-Programmed Tests
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Mulitplexed Bead ELISA's|In Cell Westerrns|In Cell Protein Quant|RFP|Gold Standard Cell Count and Viability|Mito Potential|ROS|Phagocytosis
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Resolution Histogram Bins
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1000
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Sample Type
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Beads|Cell Preparations
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Sample Volume
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75 μL
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Supported Connectivity
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USB on-the-go
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Useable Cell Volume
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14 - 2145 fL Type MF-S
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Weight
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3.18 kg
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How It Works
The operating principle behind the Moxi Go & Moxi GO II Flow Cytometers is a unique combination of Coulter-style cell size determination with simultaneous fluorescence detection. As cells flow single file through the microfabricated single-use flow cell the volume of each particle is measured at the exact same time as their primary fluorescence is measured using either a 532nm (MXG001) or 488nm (MXG002) solid state diode laser with and with the following emission filters - MXG001: 561/LP nm (e.g. PE, RFP), MXG002: User-swappable 525/45nm (e.g. FITC, GFP) or 561nm/LP (e.g. PE, RFP), MXG102: 525/45nm (e.g. FITC, GFP) and 561nm/LP (e.g. PE, RFP). Thousands of cells are measured in the 10 second read time and the data are plotted in a gradient density scatter plot as Cell size (volume) vs. Fluorescence (PMT voltage). Gating is easily performed on the unit using a interactive touch display, and the resulting live/dead ratios are automatically calculated (depending on the app selected). The analyzed data can also be displayed as a two color size histogram. Total volumetric cell counts are automatically determined for each test by precisely measuring the volume of fluid being analyzed.
Step 1:
Select desired app, insert the cassette and close the doors.
Step 2:
Once auto-alignment is complete, open the top door and pipette 75μl of labelled sample into the cassette.
Step 3:
Close the top door, assays run automatically and results are generated in ~10 seconds. Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into Moxi GO, and load Sample 2.
Data:
Data can be displayed on the unit in both a color density scatter plot and a two color size histogram. Simply drag gates using the intuitive touch display for instant live/dead ratio calculations and each of the gated volumetric cell counts (i.e., total population, live population, and dead population (Viability App). The mean cell volume for the gated populations is also automatically displayed on the unit. Results from each test are stored in the standard FCS 3:1 format and can be viewed using any flow cytometry analysis package. The actual Moxi Flow screenshots from each assay (dot plots and histograms) are also stored in bitmap format for use online. Hundreds of files can be stored on each Moxi GO and are easily transferred to a Mac or PC using USB on-the-go. No aditional software is required.
簡單介紹
世聯(lián)博研（北京）科技有限公司正式做為Orflo中國代理商，主要產(chǎn)品包括Moxi Flow微型流式細(xì)胞儀及MoxiZ細(xì)胞計(jì)數(shù)產(chǎn)品系列，迷你細(xì)胞計(jì)數(shù)儀Moxi Z和掌上流式細(xì)胞儀MOXI FLOW
產(chǎn)品描述
備有庫存現(xiàn)貨，隨時(shí)發(fā)貨
ORFLO Technologies總部位于美國愛達(dá)荷州，擁有多項(xiàng)國際**技術(shù)，如微流控、生物信號向電子信號轉(zhuǎn)化、數(shù)據(jù)采集和分析、配套分析軟件設(shè)計(jì)等，**于現(xiàn)代細(xì)胞分析技術(shù)**和開發(fā),在國際細(xì)胞分析技術(shù)競爭中處于**的地位。
ORFLOTechnologies 2010年開發(fā)****個(gè)掌上細(xì)胞計(jì)數(shù)儀，產(chǎn)品一經(jīng)推出，很快受到業(yè)內(nèi)廣泛認(rèn)可。目前推出*新代迷你細(xì)胞計(jì)數(shù)儀Moxi Z和掌上流式細(xì)胞儀MOXI FLOW。
世聯(lián)博研（北京）科技有限公司正式做為Orflo中國代理商，主要產(chǎn)品包括Moxi Flow微型流式細(xì)胞儀及MoxiZ細(xì)胞計(jì)數(shù)產(chǎn)品系列等。
配套耗材：
一、Moxi Z 微型自動(dòng)細(xì)胞計(jì)數(shù)儀配套芯片(Cell Counting Cassettes)
芯片覆蓋直徑范圍（2-34微米）內(nèi)的所有細(xì)胞樣品，無需根據(jù)細(xì)胞大小進(jìn)行更換，
每個(gè)芯片可做2個(gè)測試。當(dāng)樣品1完成后，只需取出，將芯片的另一端插入Moxi Z，加入樣品2。
在一次性盒式芯片中具有更高的性能和簡單性。
Moxi Z獨(dú)特的**保護(hù)薄膜細(xì)胞計(jì)數(shù)盒可在8秒鐘內(nèi)提供高精度，可重復(fù)的細(xì)胞計(jì)數(shù)和細(xì)胞大小分析（S型盒帶15秒）。受全球**計(jì)數(shù)系統(tǒng)使用的金標(biāo)準(zhǔn)Coulter原理的啟發(fā)，Moxi盒每個(gè)測試通過一個(gè)細(xì)胞感測區(qū)域流動(dòng)數(shù)千個(gè)細(xì)胞，以準(zhǔn)確捕獲樣品中每個(gè)單個(gè)細(xì)胞的基于阻抗的體積測量?；趫D像的細(xì)胞計(jì)數(shù)方法，單個(gè)細(xì)胞的基于阻抗的細(xì)胞計(jì)數(shù)被證明是超過95％的準(zhǔn)確度，而75％的準(zhǔn)確度。
沒有系統(tǒng)污染和集成預(yù)過濾器防止堵塞。
Moxi盒式芯片設(shè)計(jì)有獨(dú)特的“細(xì)胞篩”，以*大限度地減少細(xì)胞結(jié)塊和堵塞。另外，Moxi盒含有100％的樣品在盒體內(nèi)，消除了系統(tǒng)污染和**的可能性。
1、Moxi Z S型細(xì)胞計(jì)數(shù)芯片（Moxi Z Type S Cassettes）
The Type S cassette is ideal for accurately measuring most yeast and other particles as small as 3 μm and up to 20 μm in average diameter including some algae and protozoa. Since the technology is based on a volumetric measurement, non-spherical particles (14 - 4,200 fL) can also be measured accurately.
S型細(xì)胞計(jì)數(shù)芯片非常適用于**測量大多數(shù)酵母和其他顆粒，平均粒徑小至3μm，直徑達(dá)20μm，包括一些藻類和原生動(dòng)物。 由于該技術(shù)基于體積測量，因此也可以準(zhǔn)確測量非球形顆粒（14-4,200fL）。
Cell Counting Cassettes
Higher Performance & Simplicity in One Disposable Cassette.
Moxi Z's unique, patent-protected thin-film cell count cassettes provide highly accurate, repeatable cell counts and cell size analysis in under 8 seconds (15 seconds for Type S cassette). Inspired by the gold-standard Coulter Principle used in high-end counting systems worldwide, the Moxi cassette flows thousands of cells per test through a cell sensing zone to accurately capture the impedance-based volumetric measurement of each individual cell in the sample. Impedance-based cell counting of individual cells is proven to be over 95% accurate versus a 75% accuracy with image-based cell counting methods.
No system contamination and integrated pre-filter for clogging prevention.
Moxi cassettes are designed with a unique "cell sieve" to minimize cell clumping and clogging. In addition, Moxi cassettes contain 100% of your sample within the cassette body, eliminating the possibility for system contamination and sterilization. Cassettes are simply and easily discarded when testing is finished.
Moxi Z Type S Cassettes
The Type S cassette is ideal for accurately measuring most yeast and other particles as small as 3 μm and up to 20 μm in average diameter including some algae and protozoa. Since the technology is based on a volumetric measurement, non-spherical particles (14 - 4,200 fL) can also be measured accurately.
Moxi Z Type S Pack (25 Cassettes)
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Moxi Z Type S Box (250 Cassettes)
Moxi Z Type M Cassettes
The Type M cassette is ideal for accurately measuring mammalian cells or other particles 4-25 microns in average diameter. Since the technology is based on a volumetric measurement, non-spherical particles (34-8,180 fL) can also be measured accurately.
Moxi Z Type M Pack (25 Cassettes)
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Moxi Z Type M Box (250 Cassettes)
MOXI Z System Check Beads (5 mL)
Product Code: MXA005
MOXI Z System Check Beads (5 mL)
 Sh List
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Related Products
Accessories
Cassettes
Instruments
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id
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MXA005
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Application Notes
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Can be used to check proper system function.
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Bead Diameter
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10 micron
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Compatible Instruments
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Moxi Z
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Concentration
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1.1 e+5 beads/mL
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Formulation
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Orflo Diluent
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Intended Use Statement
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For Research Use Only. Product is not for use in diagnostic procedures
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Material of Construction
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Polystyrene
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Overall Dimensions
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22.1 mm^3
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Sterility
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Non-sterile
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Weight
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5 mg
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Moxi Z Diluent
Product Code: MXA006
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Related Products
Reagents
Accessories
Cassettes
Instruments
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id
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MXA006
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Application Notes
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Moxi Z Diluent is special formulated for use with the Moxi Z system for optimal sizing of particles and for operation with the instrument's Small Particle Mode (SPM).
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Applications
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Cell Dilution
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Applications Abbreviation
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Cdil
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Cassette Types
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Type M|Type S
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Compatible Instruments
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Moxi Z
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Formulation
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Proprietary
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Intended Use Statement
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For Research Use Only. Product is not for use in diagnostic procedures
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Kit Components
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One 100 ml bottle of diluent
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Number of Tests
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n/a
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Overall Dimensions
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52 L x 52 W x 106 H (mm)
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Protocol
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Pellet and resuspend cells in Moxi Z diluent or dilute sample with >90% Moxi Z diluent. 2. Pipette 10 uL of sample into tube 3. Pipette 90 uL of Orflo Diluent into tube 4. Gently mix resulting solution to ensure cells are evenly mixed 5. Pipette 75 uL of resulting solution into Moxi Z Cassette, or 50 uL into Moxi Flow cassette 6. Touch screen to run test
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Sample Type
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Mammalian Cells|Large Yeast|Large Algae|Protozoa
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Sterility
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Non-Sterile
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Storage Stability AND Handling
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Store at 2-8C. Use sterile technique when handling. Dispose of waste product in compliance with federal, state and local regulations. Do not inhale or ingest. Avoid contact with eyes and skin, flush affected area with water for at least 15 minutes
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Vendor
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ORFLO
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Weight
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152 g
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Moxi Z Diluent, sample
Product Code: MXA009
Moxi Z Diluent, 5 mL
See Product Specifications
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Related Products
Reagents
Accessories
Cassettes
Instruments
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id
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MXA009
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Application Notes
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Moxi Z Diluent is special formulated for use with the Moxi Z system for optimal sizing of particles and for operation with the instrument's Small Particle Mode (SPM).
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Applications
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Cell Dilution
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Applications Abbreviation
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Cdil
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Cassette Types
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Type M|Type S
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Compatible Instruments
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Moxi Z
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Formulation
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Proprietary
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Intended Use Statement
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For Research Use Only. Product is not for use in diagnostic procedures
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Kit Components
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One 5 ml bottle of diluent
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Number of Tests
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75
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Overall Dimensions
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25 W x 45 H (mm)
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Protocol
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Pellet and resuspend cells in Moxi Z diluent or dilute sample with >90% Moxi Z diluent. 2. Pipette 10 uL of sample into tube 3. Pipette 90 uL of Orflo Diluent into tube 4. Gently mix resulting solution to ensure cells are evenly mixed 5. Pipette 75 uL of resulting solution into Moxi Z Cassette, or 50 uL into Moxi Flow cassette 6. Touch screen to run test
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Sample Type
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Mammalian Cells|Large Yeast|Large Algae|Protozoa
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Sterility
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Non-Sterile
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Storage Stability AND Handling
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Store at 2-8C. Use sterile technique when handling. Dispose of waste product in compliance with federal, state and local regulations. Do not inhale or ingest. Avoid contact with eyes and skin, flush affected area with water for at least 15 minutes
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Vendor
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ORFLO
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Weight
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11 g
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Orflo Accutasea
Product Code: MXA020
Orflo Accutase cell detachment solution, 1X, 100 mL
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Related Products
Reagents
Assays
Antibodies
Cassettes
Instruments
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id
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MXA020
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Application Notes
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Recommended for the detachment of adherent cells and dissociation of mammalilian cell lines and primary cells
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Applications
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Cell Detachment
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Applications Abbreviation
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Cdet
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Cassette Types
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Type M|Type S|Type MF-M|Type MF-S
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Cell Types Tested
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fibroblasts|keratinocytes|vascular endothelial cells|hepatocytes|vascular smooth muscle cells|hepatocyte progenitors|primary chick embryo neuronal cells|bone marrow stem cells|adherent CHO and HNK cells|macrophages|293 cells|L929 cells|immortalized mouse testicular germ cells|MIC5|3T3|Vero|COS|HeLa|NT2|MG63|M24 and A375 metastatic melanoma|gliomas U251|D54|HT1080 fibrosarcoma cells|HT29 colon cancer cells|Sf9 insect cells|human embryonic stem cells|human mesenchymal stem cells|and human neural stem cells.
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Compatible Instruments
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MoxI Z|Moxi Flow|zEPI Flow
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Formulation
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Accutase® enzymes in Dulbecco's PBS containing 0.5 mM EDTA and phenol red. 1X concentrate, no preservatives.
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Intended Use Statement
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For Research Use Only. Product is not for use in diagnostic procedures
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Kit Components
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One 100ml bottle of cell dissassociation reagent
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Number of Tests
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n/a
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Overall Dimensions
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52 L x 52 W x 106 H (mm)
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Protocol
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1. Place 100ml Accutase bottle in room temp water bath or thaw overnight in fridge 2. For Adherent Cells: a. Remove cell media b. Add 1.5ml Accutase per 25cm2 of flask area c. incubate at RT or 37C until cells detach (typically 5-15min) d. Pipette triturate to break apart clusters 3. For suspension cell aggregates: a. Pellet cells at 300xg, 5min b. Resuspend pellet in 1-2ml Accutase c. Incubate 15min d. Pipette triturate to break apart clusters 4. Count cells on the Moxi Z, Moxi Flow, or Zepi Flow (be sure to dilute samples into standard operating range of specific cassette, if they are above the stated concentration limits)
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Sample Type
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Primary Mammalian cells|immortalized adherent cells|non-ES cell types
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Sterility
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Sterile
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Storage Stability AND Handling
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Stable at -20C. After thawing can be stored for up to 2 months at 2-8C.
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Tradename
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ACCUTASE? is a registered trademark of Innovative Cell Technologies
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Vendor
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ORFLO
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Weight
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159 g
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Orflo Accumaxa
Product Code: MXA021
Orflo Accumax, cell dissassociation reagent, 10X, 100 mL, sterile
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Related Products
Reagents
Assays
Antibodies
Cassettes
Instruments
|
id
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MXA021
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Application Notes
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Recommended for use with the Moxi Z and Moxi Flow platforms when working with clumpy aggregated cells. It is ideal for generating single cell suspensions from clumped cell cultures, detachment of cells from primary tissue and detachment of cells from plastic tissue culturware. Accumax is specifically designed for our combination fluorescence amp impedance based flow cytometers.
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Applications
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Cell Detachment
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Applications Abbreviation
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Cdet
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Cassette Types
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Type M|Type S|Type MF-M|Type MF-S
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Compatible Instruments
|
MoxI Z|Moxi Flow|zEPI Flow
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Formulation
|
10X proprietary solution of proteolytic, collagenolytic and DNase enzymes and does not contain mammalian or bacterial derived products.
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Intended Use Statement
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For Research Use Only. Product is not for use in diagnostic procedures
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Kit Components
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One 100ml bottle of cell dissassociation reagent
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Number of Tests
|
n/a
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Overall Dimensions
|
52 L x 52 W x 106 H (mm)
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Protocol
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1. Place 100ml Accumax bottle in room temp water bath or thaw overnight in fridge 2. For Adherent Cells: a. Remove cell media b. Add 1.5ml Accumax per 25cm2 of flask area c. incubate at RT or 37C until cells detach (typically 5-15min) d. Pipette triturate to break apart clusters 3. For suspension cell aggregates: a. Pellet cells at 300xg, 5min b. Resuspend pellet in 1-2ml Accumax c. Incubate 15min d. Pipette triturate to break apart clusters 4. Count cells on the Moxi Z, Moxi Flow, or Zepi Flow (be sure to dilute samples into standard operating range of specific cassette, if they are above the stated concentration limits)
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Sample Type
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Primary Mammalian cells|immortalized adherent cells|Human ES cells|Rodent ES
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Sterility
|
Sterile
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Storage Stability AND Handling
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Stable at -20C. After thawing can be stored for up to 2 months at 2-8C.
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Tradename
|
ACCUMAX? is a registered trademark of Innovative Cell Technologies
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Vendor
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ORFLO
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Weight
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159 g
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