CleanPlex®神經(jīng)遞質(zhì)紊亂面板是一種預(yù)先設(shè)計(jì)和定制的基于多重PCR /擴(kuò)增子的靶向測(cè)序（NGS）分析方法，旨在檢查與神經(jīng)遞質(zhì)紊亂相關(guān)的22個(gè)基因的種系變異或突變。該小組針對(duì)這些基因的所有外顯子區(qū)域和側(cè)翼內(nèi)含子序列。僅需10 ng DNA即可兼容測(cè)序就緒的文庫(kù)，只需3個(gè)小時(shí)即可使用簡(jiǎn)化的工作流程進(jìn)行準(zhǔn)備。預(yù)先設(shè)計(jì)的面板經(jīng)過(guò)計(jì)算機(jī)優(yōu)化，可提供具有高目標(biāo)性能和高覆蓋均勻性的數(shù)據(jù)，以確保有效利用測(cè)序讀數(shù)。
該產(chǎn)品是定做的。收到您的訂單后，我們將合成面板，該套件將包含CleanPlex Multiplex PCR引物和CleanPlex Targeted Library Kit。可以分別訂購(gòu)CleanPlex索引PCR引物和CleanMag®磁珠，以完成從輸入DNA到可測(cè)序的NGS文庫(kù)的工作流程。
Gene List: ABAT, ALDH5A1, ALDH7A1, ARHGEF9, DBH, DDC, GAD1, GCH1, GLDC, GLRA1, GLRB, GPHN, MAOA, PCBD1, PHGDH, PNPO, PTS, QDPR, SLC6A3, SLC6A5, SPR, TH References: Mei D, et al. The Impact of Next-Generation Sequencing on the Diagnosis and Treatment of Epilepsy in Paediatric Patients. Mol Diagn Ther. 2017 Aug;21(4):357-373. Narwade SC, et al. Transcriptome Analysis Reveals Altered Expression of Memory and Neurotransmission Associated Genes in the REM Sleep Deprived Rat Brain. Front Mol Neurosci. 2017 Mar 17;10:67.
Gene List: ABAT, ALDH5A1, ALDH7A1, ARHGEF9, DBH, DDC, GAD1, GCH1, GLDC, GLRA1, GLRB, GPHN, MAOA, PCBD1, PHGDH, PNPO, PTS, QDPR, SLC6A3, SLC6A5, SPR, TH
References: Mei D, et al. The Impact of Next-Generation Sequencing on the Diagnosis and Treatment of Epilepsy in Paediatric Patients. Mol Diagn Ther. 2017 Aug;21(4):357-373.
Narwade SC, et al. Transcriptome Analysis Reveals Altered Expression of Memory and Neurotransmission Associated Genes in the REM Sleep Deprived Rat Brain. Front Mol Neurosci. 2017 Mar 17;10:67.